油菜NADH脱氢酶亚基9的生物信息学分析
关键词：油菜；NADH脱氢酶亚基9蛋白；生物信息学
Bioinformatic analysis of NADH dehydrogenase subunit 9 protein in Brassica napus L
Abstract:NADH dehydrogenase, is the first enzyme of the mitochondrial electron transport chain, NADH dehydrogenase subunit 9 is NADH dehydrogenase in the IX peptide chain. This article aims to analyze basic physical and chemical properties, secondary structure and tertiary structure modeling of NADH dehydrogenase subunit 9 in Brassica napus L using a bioinformatics approach to understand the related information between structure and function. The results showed that the protein, containing 190 amino acids, has higher similarity to homology protein of other species. There has not transmembrane helix region and signal peptide sequence in AA sequence, is secreted proteins. It comprises a plurality of different phosphorylation sites and located in the cytoplasm. There is a small disorder area and the larger potential globulin area forming in the 3-138 and 147-186. Furthermore, the tertiary structure and surface potential model of the protein was built. This study provided the reference for depth study structure and biological function of NADH dehydrogenase subunit 9 in Brassica napus L.
Key Words: Brassica napus L; NADH dehydrogenase subunit 9; Bioinformatics
目 录
摘 要 1
引 言 2
1.材料和方法 2
1.1 材料来源及序列 2
1.2 生物信息学分析软件 3
2.结果与分析 4
2.1 油菜与其他植物的NADH脱氢酶亚基9的同源性分析 4
2.2 基本理化性质分析 5
2.3 油菜NADH脱氢酶亚基9的疏水性 6
2.4 信号肽分析、跨膜预测、亚细胞定位和磷酸化分析 7
2.5 二级结构、蛋白紊乱区、球蛋白区预测 9
2.6 油菜NADH脱氢酶亚基9蛋白的模体预测 10
2.7 油菜NADH脱氢酶亚基9蛋白的同源建模结果和评价 11
2.8 NADH脱氢酶亚基9蛋白的表面静电构像 13
3.讨论与结论 14
参考文献 14
致 谢 15
油菜NADH脱氢酶亚基9的生物信息学分析引 言
线粒体复合物I(NADH:ubiquinone oxidoreductase) 由NADH脱氢酶（一种以FMN为辅基的黄素蛋白）和一系列铁硫蛋白（铁-硫中心）组成。除了酵母等低等真核生物外，复合物I在绝大多数真核生物中是一个很复杂的线粒体电子呼吸链的元件。它在糖酵解、糖异生、三羧酸循环及呼吸链中发挥着不可替代的作用，其中间产物会将脱下的氢递给NAD，使之成为NADH，代谢产生的NADH经由电子传递氧化磷酸反应可产生大量ATP[1]。在真核生物线粒体中最小的复合物I由14种保守的亚基组成，而在植物中有超过40种亚基组成[2]。这些亚基大部分由核基因编码后在胞质中转录，然后转运到线粒体。在哺乳动物中的7种亚基(Nad 1～Nad6、Nad4L )和在植物的9种亚基(Nad1～Nad7、Nad4L和Nad9 )均由线粒体编码[3,4]。
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