癌症是医学难题，其早期诊断研究意义重大。荧光光谱分析法灵敏度高，快速，样品获取和处理过程相对简便。血清荧光谱中携带有癌症相关信息，其研究受到很大重视，在早期癌症诊断方面的应用潜力被寄予希望。然而，现有研究在诊断指标的量化表示、和特异性等方面的进展尚未能满足人们的要求。68252

本文通过实验检测获取肝癌小鼠和健康小鼠血清的荧光光谱，研究其谱线结构特征及其与肿瘤的相关性。主要分析了激发光波长为280 nm的光谱数据，所研究的光谱曲线在300-400 nm波段具有展宽的单峰曲线型结构。

本文的研究结果表明，上述单峰曲线的构型与接种于小鼠的肿瘤生长的天数显著相关，表现为随接种肿瘤的生长发展，荧光谱曲线的单峰线型轮廓向短波长方向倾斜，其峰值波长（峰位）向短波方向有显著偏移（蓝移）；论文分别提出了以峰值波长偏移量和一阶导数积分指数来量化表示上述“倾斜”的方法，由实验数据的统计分析表明，此峰位偏移量和一阶导数积分指数都能够在一定层面上量化反应小鼠肿瘤的发生和发展过程，而一阶导数积分指数的动态范围更大，对数据检测的精度要求可以更低。

本文的研究方法和结果，可以为进一步发展新的癌症诊断方法和技术，提供有价值的参考。

毕业论文关键词：血清，荧光光谱，肿瘤，导数，积分指数

毕业设计说明书（论文）外文摘要

Title A study in wavelength of 280nm laser excitation fluorescence spectra obtained by the serum of liver cancer in mice

Abstract

Cancer is a challenge in the medical field. Diagnosis of incipient cancer is of great significance. With high sensitivity, high speed and relatively simple acquisition and processing of sample, fluorescence spectrum is an effective method in the analysis of material composition and molecular structure. The detection and analysis methods of fluorescence spectra into the tumor detection are of high hopes, however there are some difficulties to be solved.

This assay, the fluorescence spectra of the serum of healthy mice and liver cancer in mice, analysis of the characteristics of its spectrum under different excitation wavelength of peak fluorescence intensity and peak frequency shift changes in the relationship. Excitation wavelength of 280 nm spectral data, the study of spectral curves in the 300-400 nm band broadening unimodal structure.

The results of this study showed that the single- peak curve configuration and tumor growth in mice inoculated in the number of days was significantly associated with tumor inoculation showed the growth of a single peak of the fluorescence spectrum curve linear contours tilted towards shorter wavelengths , the peak wavelength ( peak ) to a significant shift shorter wavelength ( blue shift ); thesis presents the peak wavelength shift amount and the first derivative of the integral index quantifies the  "tilt " method , statistical analysis of the experimental data showed that, this peak position offset and a credit derivative indices are able to react in a certain level of quantification mouse tumor occurrence and development process, and a derivative score index greater dynamic range , the quantization effect is more pronounced , more suitable for tumorigenesis discriminant.

Methods and results of this study can be for the further development of new cancer diagnostic methods and technology to provide a valuable reference

Keyword: serum, fluorescence spectrum, cancer, first derivative, integral exponential  

目次

1 引言 3

1.1 研究背景与意义