摘要：研究目的：研究和探讨小鼠肝损伤后对花青素代谢是否有影响。研究方法：0。3%四氯化碳灌胃小鼠进行肝损伤造模，建模后对小鼠进行灌胃花青素并采用眼球取血法在不同时间点取血,分别离心后取上清，用高效液相色谱（HPLC）分析血液内的花青素含量变化，并与正常小鼠、离体血液和生理盐水中加入的花青素进行比较。 研究结果：通过观察液相色谱图得到花青素样品为复合物。比较花青素在离体血液和生理盐水中随时间的变化花青素并没有大量降解，只有很少一部分被降解。同时花青素在小鼠肝脏代谢的实验证明小鼠肝脏能够大量代谢花青素组分A和组分B，花青素组分A的代谢主要依赖肝脏，花青素组分B的代谢对肝脏的依赖性较小。并且比较正常小鼠和肝损伤小鼠对花青素的代谢结果得到：肝损伤的小鼠比正常小鼠代谢花青素速度减缓。74921

毕业论文关键词：肝损伤模型建立；高效液相色谱（HPLC）；花青素；药代动力学

Effect of mouse liver injury on the pharmacokinetics of anthocyanins

Abstract: Objective: This study is about whether liver damage has effect on anthocyanin metabolism。 Research method: liver damage model was made by mice which were taken 0。3% carbon tetrachloride by intragastric administration。 After modeling, the mice were taken anthocyanin by intragastric administration, then sampling blood in eyes at different time point。 Centrifuging，supernatant was made as samples。 Determination of anthocyanin content in samples by high performance liquid chromatography (HPLC) and compare it with the anthocyanin content from normal mice, isolated blood and saline。 Research result: The standard anthocyanin chromatography shows that anthocyanin is composited。 Also, anthocyanin content of isolated blood and saline shows that the degradation of anthocyanin is not much。 In the meantime, anthocyanin in mice liver metabolism experiments proves that mice liver can metabolize composition A and B of anthocyanin, and the composition A of anthocyanin rely mainly on liver。 However, composition B is less dependent。 Compared with normal mice, anthocyanin metabolism of liver damaged mice has a delay。

Keywords: Liver injury; HPLC; anthocyanin; Pharmacokinetics 

目录

引言 1

1 材料与方法 2

1。1 供试材料 2

1。1。1实验材料： 2

1。1。2实验小鼠选取： 2

1。1。3实验试剂： 2

1。1。4实验仪器： 2

1。2。实验方法 3

1。2。1花青素在小鼠离体血液里的药代动力学分析 3

1。2。1。1配制试剂： 3

1。2。1。2小鼠眼球取血 3

1。2。1。3 取血后分离血清 3

1。2。2花青素在生理盐水中的药代动力学分析 4

1。2。2。1配制药品： 4

1。2。2。2 高效液相色谱（HPLC）检测分析 4

1。2。3花青素在肝损伤小鼠体内的药代动力学分析 4

1。2。3。1配制试剂： 4

1。2。3。2 小鼠急性肝损伤模型的建立： 5

1。2。3。3 花青素灌胃给药：