呕吐毒素对仔猪肝脏和小肠组织形态的影响及脱霉剂效果观察

摘要：为探究呕吐毒素(DON)对仔猪肝脏、小肠组织形态的影响及脱霉剂使用效果，试验选取96头健康大白×长白二元杂交仔猪，依初始体重相近、公母各半为原则随机分为4组，分别为对照组、对照组+脱霉剂组、DON 3000组和DON 3000+脱霉剂组，每组3个重复，每个重复8头仔猪。仔猪适应环境一周后，开始正式试验。每组饲喂相应的试验日粮，饲养3周后，每组选取3只仔猪屠宰并采集相应组织和器官。制作石蜡切片，应用H.E.染色法检测仔猪肝脏、小肠组织形态。结果显示，呕吐毒素造成小肠绒毛顶端受损、脱落，固有层萎缩，肠道上皮细胞数量较少，造成肝细胞出现局部空泡变性，肝血管管壁增厚。脱霉剂起到相应的缓解效果，减少呕吐毒素对仔猪肠道的组织结构损伤。研究结果为进一步研究呕吐毒素对仔猪肝脏、小肠组织形态影响及脱霉剂效果提供试验依据。37287
毕业论文关键词：呕吐毒素；肝脏；脱霉剂；小肠；H.E.染色
Effects of deoxynivalenol and remove agent on the morphology of liver and small intestine in piglets
Abstract： In order to investigate the effect of deoxynivalenol (DON) and remove agent on the morphology of liver and small intestine in piglets. Ninety-six piglets(commercial Landrace - Yorkshire crossbred) were chosen at 5-week-old. Piglets were randomly pided into 4 groups according to the similar initial weight and sex. These four groups were Control group, Control + remove agent group, DON 3000 group, and DON 3000 + remove agent group. There were three replications per group and eight piglets per replicate. Piglets were allowed to acclimate at least 1 week prior to beginning experiment. After three weeks each group was selected three piglets to slaughter and collect the corresponding tissues and organs of 3 piglets. Paraffin sections were used to detect the morphology of liver and small intestine in piglets by hematoxylin-eosin staining. The results showed that DON could induce the damage to the liver and small intestine. The removal agent relieves the toxicity of DON to piglets and reduces the intestinal injury caused by vomiting toxin.The results provide an experimental basis for the further study of the effects of emetic toxin on the morphology of liver and small intestine in piglets.
Key words: DON；liver；small intestine；removal agent；hematoxylin-eosin staining
目录
摘要2
关键词2
Abstract2
Key words2
引言(或绪论)2
1 材料与方法3
1.1 试验材料3
1.1.1 主要仪器 3
1.1.2 主要试剂3
1.2 研究方法3
1.2.1 试验分组4
1.2.2 样品处理4
1.2.2.1 制作石蜡块4
1.2.2.2 H.E.染色4
2 结果与分析 5
2.1 H.E.染色结果分析5
2.1.1 小肠组织染色结果分析5
2.1.2 肝脏组织染色结果分析5
3 讨论6
3.1 DON对仔猪肝脏，小肠组织形态的影响6
3.2 脱霉剂效果影响6
4 结论7
致谢7
参考文献8
图1. 仔猪小肠组织H.E.染色切片５
图2. 仔猪肝脏组织H.E.染色切片６
呕吐毒素对仔猪肝脏和小肠组织形态的影响及脱霉剂效果观察
脱氧雪腐镰刀菌烯醇(deoxynivalenol，DON)，又称为呕吐毒素，一般在玉米、大麦、小麦中的污染较为严重，是最为常见的一种真菌毒素，主要是由黄色镰刀杆菌和禾谷镰刀菌产生的一类生物活性和化学结构相似的一种次级代谢产物，属于单端孢霉烯族毒素。据统计，全球有25%的作物被真菌毒素污染[1]。低浓度的DON会引起动物的体增重降低，厌食，营养物质利用率降低等慢性中毒症状；高浓度的DON易导致腹泻，呕吐，免疫功能异常，甚至会出现死亡等急性中毒症状[2-5]。研究表明，DON不仅会造成动物各种器官毒性、生殖系统毒性、细胞毒性及免疫毒性[6]，严重危害动物健康，还可以通过食物链危害人类健康。呕吐毒素对仔猪肝脏和小肠组织形态的影响及脱霉剂效果观察:http://www.youerw.com/yixue/lunwen_36021.html