曲克芦丁抑制BDE-47诱导的小鼠肝脏细胞凋亡

摘要目的：利用四溴联苯醚(2,2′,4,4′-tetrabromodiphenyl ether，BDE-47) 建立小鼠肝脏损伤模型，本文探讨曲克芦丁对BDE-47诱导的小鼠肝脏细胞凋亡的保护作用和分子机制。方法：建立BDE-47诱导的小鼠肝脏损伤模型，再用曲克芦丁（troxerutin）处理，设置Control组、BDE-47（150mg/kg/day）组、BDE-47（150mg/kg/day）+ Troxerutin （150mg/kg/day）组和Troxerutin（150mg/kg/day）组共四组，连续喂养12周后处死。（1）以生化检测技术对小鼠肝脏氧化应激指标进行检测。（2）以Western blot法对小鼠肝细胞凋亡相关通路指标进行检测。结果：（1）BDE-47处理后小鼠肝脏细胞中自由基含量明显升高，曲克芦丁处理后使得肝脏中自由基含量明显减少。（2）BDE-47处理后小鼠肝脏细胞内质网中P-PERK、P-eIF2含量显著升高，曲克芦丁处理后使得肝脏细胞内质网中P-PERK、P-eIF2含量明显减少。（3）BDE-47处理后小鼠肝脏细胞凋亡相关蛋白Cleaved-Caspase-3、GADD34和Bax含量均显著升高，抗凋亡蛋白Bcl-2含量明显减少。曲克芦丁处理使得小鼠肝脏细胞凋亡相关蛋白Cleaved-Caspase-3、GADD34和Bax含量明显减少，抗凋亡蛋白Bcl-2含量明显升高。结论：曲克芦丁能够通过抑制BDE-47诱导的小鼠肝脏氧化应激，进而抑制肝脏内质网应激从而抑制肝脏细胞凋亡。50725

该论文有图3幅，表1个，参考文献21篇。

毕业论文关键词：2,2′,4,4′-四溴联苯醚曲克芦丁细胞凋亡内质网应激

Troxerutin Inhibits BDE-47-induced Apoptosis in Mouse Liver

Abstract

Objective：In this study, mouse model of liver injury induced by 2, 2 ', 4, 4 '- tetrabromodiphenyl (BDE -47) was established to explore the protective effects of troxedrtutin on apoptosis in mouse liver. Methods: 24 mice were pided into four groups: Control group, BDE-47 group (150mg/kg/day), BDE-47(150mg/kg/day) +Troxerutin group (150mg/kg/day)and the Troxerutin (150mg/kg/day). After 12 weeks of treatment, mice were sacrificed, then serum ALT level, hepatic ROS level and the protein levels of P-PERK, P-eIF2, Cleaved Caspase-3, the Bcl-2 and GADD34 were determined.Results: BDE-47 treatment induced a significant increase in the levels of serum ALT, hepatic ROS and the protein levels of P-PERK, P-eIF2, Cleaved Caspase - 3 and GADD34, and a notable decrease in Bcl-2 level in mice. Nevertheless, troxerutin markedly decreased the levels of serum ALT, hepatic ROS, the protein levels of endoplasmic reticulum stress markers and apoptosis-related protein, and restored the protein level of Bcl-2 in BDE-47-treated mice. Conclusion: Troxerutin could protect against BDE-47-indued apoptosis via inhibiting oxidative stress, thereby attenuating ER stress, furthermore suppressing apoptosis-related signaling in mouse liver.

Key Words: BDE-47 troxerutin apoptosis endoplasmic reticulum stress

摘要-Ⅰ

Abstract-Ⅱ

目录Ⅲ

图清单Ⅳ

表清单Ⅳ

变量注释表--Ⅳ

1绪论--1

2材料与方法-1

2.1实验动物-1

2.2实验试剂-2

2.3实验仪器-2

2.4实验方法-2

3实验结果分析-3

3.1曲克芦丁对BDE-47诱导的小鼠肝脏损伤的保护作用-4

3.2曲克芦丁对BDE-47诱导的小鼠肝脏氧化应激的保护作用-4

3.3曲克芦丁对BDE-47诱导的小鼠肝脏内质网应激的保护作用-4

3.4曲克芦丁对于BDE-47诱导的小鼠肝脏细胞凋亡的保护作用-5

4讨论6

5参考文献--7

6致谢8

图清单

图序号图名称页码

图3-2 肝脏自由基含量曲克芦丁抑制BDE-47诱导的小鼠肝脏细胞凋亡:http://www.youerw.com/yixue/lunwen_54093.html