光学显微相位成像在细胞形态检测中的应用研究

摘要定量干涉显微测量是一种高精度、无损、实时的生物样品相位计量技术。为了能够从干涉图中恢复被测生物样品的定量相位分布，提出了许多相位恢复算法，如基于快速傅里叶变换的相位恢复算法，基于希尔伯特变换的相位恢复算法等等。然而这些算法都需要使用相位解包以及后续的相位消倾斜来处理由于相位提取中的反正切带来的相位间断。本文提出了一种免解包的相位恢复算法，在离轴干涉图的基础上，在实验前或者实验后多采集一张背景干涉图，通过附加的背景干涉图的信息，可以仅使用相位提取算法得到生物细胞的精确的相位分布。相对于传统算法，该算法有许多优势：其可以实现实时的生物测量，只需在测量过程之外采集一张背景干涉图；并且不需要繁复的相位解包以及相位消倾斜来处理提取相位的不连续；该方法计算时间短，可以将计算效率提高一至两个数量级；同时，该方法还能够消去由于相位提取算法或者成像系统带来的背景相位。该方法有望用于定量干涉显微系统中以实现对生物样品的实时定量显微测量。64382

毕业论文关键字定量干涉显微测量干涉图相位恢复免解包

毕业设计说明书（论文）外文摘要

Title Biological cell morphology detection with optical phase microscopy

Abstract Quantitative interferometric microscopy could be used as quantitative phase measurement of biological samples with characteristics of high-accuracy, non-invasive and real time. In order to obtain the quantitative phase distributions of biological samples, a wealth of phase retrieval algorithms have been proposed as fast Fourier transform based phase retrieval algorithms and Hilbert transform based phase retrieval algorithms, etc. However, phase unwrapping and phase tilting canceling are often needed to eliminate phase discontinuities induced by arctangent function in phase extracting. In this thesis, I have realized a phase unwrapping free phase retrieval approach which could recover phase distributions with single off-axis interferogram combining a background interferogram captured before or after the biological sample testing. Compared to traditional phase retrieval methods, the proposed method could realize real time detecting since background interferogram could be obtained outside the measuring process. The quantitative phase distribution could be obtained only with phase extracting. Since phase wrapping is avoided, the time consuming of phase recovery is much less than traditional method. The calculating efficiency could be raised by to 2 orders. Besides, this method could also cancel the phase distortions induced by phase retrieval algorithms and aberrations in imaging system. The quantitative interferometric microscopy based on phase unwrapping free phase retrieval approach is shedding light on their future directions and applications for basic and clinical research.

Keywords Quantitative interferometric microscopy Interferogram Phase retrieval Unwrapping free

1 绪论1

1.1 研究背景..1

1.2 传统生物样品测量方法..1

1.2.1 传统显微镜..1

1.2.2 荧光显微镜.2

1.2.3 相位显微镜..3

1.2.4 超分辨率显微镜4

1.2.5 光学相干层析断层技术4

1.2.6 流式细胞仪5

1.3 定量相位测量技术简介5

1.4 本文的主要工作7

2 定量显微干涉测量方法及原理.7

2.1 干涉测量原理.7

2.1.1 同轴干涉 8

2.1.2 离轴干涉.9

2.2定量显微干涉测量方法的研究进展.10

2.3定量显微干涉测量的应用简介..12

2.4 本章小结..15

3 相位恢复算法原理简介15

3.1相位提取15

3.1.1 移项法进行相位提取..15 光学显微相位成像在细胞形态检测中的应用研究:http://www.youerw.com/zidonghua/lunwen_71527.html