鲫鱼心源维隆气单胞菌的分离与鉴定(3)_毕业论文

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鲫鱼心源维隆气单胞菌的分离与鉴定(3)


1.2.8TA克隆及测序
在PCR、琼脂糖凝胶电泳、胶回收后,接着连接0.25ulpMD18-T载体,转化                                                                                                                                                                                                                                                                                                                  大肠埃希菌 DH5α,然后筛选阳性克隆,送至上海生工检测基因顺序。 (责任编辑:qin)