摘要近年来高效发光材料在电子、生物、医药等领域都具有潜在的应用价值。荧光材料由于 具有灵敏度高、选择性好、操作简捷、费用低、响应迅速、检出限低等突出的优点，在生命 科学、材料科学、环境科学、信息科学等众多领域显现出良好的应用前景，也成为了当前检 测、探明生命体系中各个重要物种非常重要并且行之有效的方法。目前，大部分传统荧光材 料存在(Aggregation-caused quenching，ACQ)效应，其水溶性、生物相容性差，细胞透过性 不好，因此不能广泛应用于化学/生物传感器、生物细胞活体成像。鉴于此，本论文基于四 苯基乙烯设计合成了一种高灵敏度且具有（Aggregation-induced emission，AIE）效应的荧光 高分子材料，应用于生物细胞活体成像。88909

通过烷基链修饰的四苯基乙烯（TPE）与苯共聚并离子化之后，得到青色荧光高分子材 料 PBTPE+。通过荧光材料对细胞毒性测试，联合共聚焦激光扫描显微成像（CLSM）和基 于时间关联单光子计数（TCSPC）技术的荧光寿命显微成像来观察经探针分子标记的 HeLa 细胞。结果表明，PBTPE+具有较低的细胞毒性及良好的生物相容性，可以进入 HeLa 细胞 并发生聚集，显示出较亮的荧光发射和不同的荧光寿命分布。

毕业论文关键词：荧光高分子材料；聚集诱导发光；四苯基乙烯；细胞成像；

Abstract Recently, high efficient luminescent materials show great potential application values in electronics, biotechnology, medicine fields, etc。。 Owing to its high sensitivity, good selectivity, convenient operation, low cost, rapid response and low detection limit, the fluorescent material has been widely applied in many fields such as biology, materials science, environmental science, information science, etc。。 It’s also a very prominent and effective method of detecting and exploring biosystem of each important species nowdays。 At present, most of the traditional fluorescent materials exhibit aggregation-caused quenching (ACQ) effect due to their poor water-solubility, biocompatibility and cell permeability。 Therefore, they could not be widely used in chemical/biosensor and biological cell imaging。 In this paper, a highly sensitive fluorescent polymer material with aggregation-induced emission  (AIE) effect containing tetraphenylethene units in the conjugated backbones were designed and synthesized。 The new AIE material was applied to the imaging of biological cell in vivo。

Copolymerizing alkyl chain-modified tetraphenylethene (TPE) with benzene to obtain neutral polymer, then treated with trimethylamine to afford cyan fluorescent polymer material PBTPE+。 Its utilities as bioprobe to detect HeLa cells were investigated through Confocal Laser Scanning Microscopy (CLSM)  and time-resolved fluorescence measurements and imaging using the Time Correlated Single Photon Counting (TCSPC) technique。 The results showed that the PBTPE+ with low cytotoxicity and good biocompatibility can enter living cells in a noninvasive manner, and the aggregated fluorescent clusters in the cells emit bright fluorescence and display different fluorescence lifetime。

Keyword: fluorescent polymer materials; aggregation-induced emission; tetraphenylethene; cell imaging

目录

1、 引言 6

1。1 基于 AIE 效应的荧光探针设计机制及方法 7

1。2 聚集诱导发光源Y于U优I尔O论P文W网wwW.yOueRw.com 原文+QQ75201-8766 （AIE）效应的机理 8

1。3 基于 AIE 效应的高分子荧光探针的应用研究