摘要：本课题以汾酒、红星二锅头酒、杏花村世家酒为代表的清香型白酒为主要 研究对象。首先，通过顶空固相微萃取法（SPME）对清香型白酒进行预处理实验， 再通过二维气相色谱质谱逐个定性定量地分析白酒中的香气成分。实验结果表 明：汾酒测出香气成分 255 种，红星二锅头酒测出香气成分 250 种，杏花村世家酒测出香气成分 218 种。其中，含量最多的是酯类化合物，还有醇类、酮类、酸 类、醛类、内酯类、含氮化合物、含硫化合物和乙缩醛类化合物。66250

关键字：汾酒；红星二锅头酒；杏花村世家酒；顶空固相微萃取法；二维气相色 谱质谱

Identification of Aroma Components in Light Aroma Type Liquor by Two - dimensional Gas Chromatography - Mass Spectrometry

Abstract ： As the representative of the light aroma type liquor ,Fenjiu, Red Star Erguotou wine, Xinghua village shijia wine are the main research objects of the subject.Firstly, the pretreatment experiment was carried out by the solid phase micro extraction process(SPME), and the aroma components in the liquor were analyzed qualitatively   and   quantitatively   by   two-dimensional   gas   chromatography-mass

spectrometry.The results showed that there were 255 kinds of aroma components in Fenjiu, 250 kinds of aroma components were detected in Hongxing Erguotou wine, and 218 kinds of aroma components were measured in Xinghua village shijia wine.Among them, the most content is the ester compounds, as well as alcohols,

ketones, acids,aldehydes,lactones, nitrogen compounds, sulfur compounds and acetal compounds.

Key words：Fenjiu；Red Star Erguotou wine；Xinghua village shijia wine；Solid phase

micro extraction process;Two-dimensional gas chromatography-mass spectrometry

目录

1 绪论 1

1.1 白酒的介绍 1

1.2 白酒香气的介绍 1

1.3 白酒样品的萃取 2

1.3.1 顶空固相微萃取的原理 2

1.3.2 顶空固相微萃取的特点 2

1.4 白酒香气成分的鉴定 2

1.4.1 二维气相色谱质谱的原理 2

1.4.2 二维气相色谱质谱与一维气相色谱质谱的对比 3

1.5 白酒香气成分的分析方法 4

1.5.1 内标法的原理 4

1.5.2 内标物的条件 4

1.6 本课题的研究目的和意义 4

1.7 本课题国内外研究现状概述 5

1.8 本课题的主要内容 6

2 实验材料和方法 7

2.1 实验材料 7

2.1.1 实验原料及试剂 7

2.1.2 实验仪器 7

2.2 实验方法 8

2.2.1 顶空固相微萃取 8

2.2.2 二维气相色谱质谱 8

2.2.3 内标法 8

3 结论与讨论 10

3.1 对白酒样品中的香气成分定性 10

3.1.1 汾酒 10

3.1.2 红星二锅头酒