摘要本实验以徐州国祯污水处理厂污泥为研究对象，通过添加食苯芽孢杆菌和恶臭假单胞菌在不同温度下对污泥进行发酵，然后采用BCR连续提取法，对污泥中Zn、Hg和Cu等8种重金属元素的4种形态进行测定，分析金属生物有效性的变化。实验结果表明：发酵30d更有利于降低重金属的生物有效性。在20℃时，锌、镍、铜、砷和镉经两种菌发酵后，金属的生物有效性降低。铬与铅经恶臭假单胞菌发酵后，有效性增加，而经食苯芽孢杆菌发酵后，铬有效性降低。在30℃时，铬、铅和汞添加两种菌发酵后，有效性降低。锌、镍和铜经恶臭假单胞菌发酵后，有效性增加，而经食苯芽孢杆菌发酵后，生物有效性降低。综上所述，在20℃和30℃时，添加食苯芽孢杆菌发酵30d后，能普遍降低重金属的生物有效性。69989

该论文有图8幅，表24个，参考文献9篇。

毕业论文关键词：城市污泥  好氧发酵  重金属  生物有效性

Bioavailability Analysis of Heavy Metals in the Sludge Aerobic Fermentation Process

Abstract This study took the sludge of Xuzhou Guozhen sewage treatment plant as the research object. The sludge fermentation experiment was conducted under different temperature condition, adding fresh benzene bacillus and pseudomonas putida into sludge, and then the method of BCR sequential extraction was used to measure the 4 forms of 8 kinds of heavy metal elements, namely Zn, Hg and Cu et.al, to analyze the change of metal bioavailability. The experimental results showed that the bioavailability of heavy metals can be effectively reduced after fermentating for 30 days. At 20℃, the bioavailability of Zn, Ni, Cu, As and Cd can be reduced with adding above two kinds of bacteria. The bioavailability of Cr and Pb were increased when adding pseudomonas putida, but the bioavailability of Cr was reduced by adding feed benzene bacillus. At 30℃, the availability of Cr, Pb, and Hg were reduced by adding two kinds of bacteria fermentation. The bioavailability of Zn, Ni and Cu were increased after adding pseudomonas putida, but the case was reduced under adding fresh benzene bacillus. Above all, the bioavailability of heavy metals can be generally reduced by fermentation adding fresh benzene bacillus for 30 days at 20℃ and 30℃.

Key words：municipal sludge  aerobic fermentation  heavy metals  bioavailability 

目  录

摘要 I

Abstract II

目  录 III

图清单 IV

表清单 IV

1前言 1

2 实验部分 2

2.1实验试剂 2

2.2 仪器设备 2

2.3 实验步骤 2

3 实验结果与分析 3

3.1 锌的有效性变化分析 3

3.2 铬的有效性变化分析 5

3.3 镍的有效性变化分析 7

3.4 铜的有效性变化分析 9

3.5 砷的有效性变化分析 11

3.6 镉的有效性变化分析 13

3.7 铅的有效性变化分析 15

3.8 汞的有效性变化分析 17

3.9各重金属有效性分析 19

4 结论