摘要厌氧氨氧化工艺在废水脱氮领域具有重要的应用前景。但厌氧氨氧菌生长过慢，倍增时间长且较易受废水中的共存污染物抑制，影响厌氧氨氧化工艺的工程应用。富集培养成功的厌氧氨氧污泥仍然易受到重金属如Cr(VI)对厌氧氨氧菌活性或其他特性的短期与长期不利影响。以血清瓶进行批次试验，分别投加0、0。5、2、5、10、30、80、160 mg L-1的Cr(VI)并进行试验,并利用非竞争性抑制模型，计算出各浓度下的抑制率即对厌氧氨氧化反应的IC20、IC50并确定Cr(VI)的最大耐受剂量MTD。通过抑制剂暴露试验与饥饿试验，考察Cr(VI)暴露时间与饥饿对厌氧氨氧化颗粒污泥有何影响。当Cr(VI)浓度为5。0 mg L-1的时候，总活性为317。93 mg/(gVSS d),当Cr(VI)浓度增加到80。0 mg L-1的时候，总活性219。90 mg/(gVSS d),当Cr(VI)浓度增加到240。0 mg L-1的时候，总活性170。30 mg/(gVSS d)。表明Cr(VI)浓度越大，厌氧氨氧化颗粒污泥的活性越低。当Cr(VI) 浓度为60 mg L-1的时候，饥饿24h的总活性为271。24 mg/(gVSS d），活性残留率为83。4%。饥饿48 h的总活性为253。83 mg/(gVSS d），活性残留率为78%，饥饿72 h后的总活性为83。65 mg/(gVSS d），其活性残留率为0。3%。与饥饿48h相比，其总活性和活性残留率有了明显的下降。这说明在同一Cr(VI)浓度投加组，其活性残留程度与饥饿程度保持一致，即饥饿时间越长，活性残留量越低。该趋势说明与Cr(VI)浓度相比，饥饿程度是对厌氧氨氧化菌更重要的影响因素。72378

Abstract Anaerobic ammonia oxidation process in wastewater denitrification field has important application prospect。But the anaerobic ammonia oxidation bacteria is difficult to culture and is more susceptible to inhibition of coexisting     pollutants in wastewater, engineering application effect of anaerobic ammonia oxidation process。Enrichment culture of anaerobic ammonia oxidation sludge successfully still vulnerable to heavy metals such as Cr(VI) on the activity of anaerobic ammonia oxidation bacteria or other characteristics of short-term and long-term adverse effects。Batch test in serum bottle, dosing respectively 0、0。5、2、5、10、30、80、160 mg L-1of Cr(VI) and experiment。And the use of non-competitive inhibition model to calculate the IC20 inhibition rate at each concentration, that is anaerobic ammonia oxidation reaction, IC50 and determine Cr (VI) of the maximum tolerated dose MTD。By the time of exposure and starvation test inhibitors investigated Cr (VI) exposure time and the impact on hunger anammox granular sludge。When the Cr (VI) at a concentration of 5。0 mg L-1, the total activity was 317。93 mg / (gVSS d), when the Cr (VI) concentration increased to 80。0 mg L-1  the total activity was 219。90 mg / (gVSS d ), when the Cr (VI) concentration increased to 240。0 mg L-1  the total activity was 170。30 mg / (gVSS d)。The results showed that Cr (VI) concentration, the lower the anaerobic ammonia oxidation activity of granular sludge。When the Cr (VI) concentration of 60 mg L-1, when hunger 24h the total activity was 271。24 mg / (gVSS d), the  activity of residual rate was 83。4%。 Hunger 48 h the total activity  was 253。83 mg / (gVSS d), the  activity of  residual rate was 78% 。 Hunger 72 h the  total activity was 83。65 mg / (gVSS d), the activity of  residual rate was 0。3%。 Compared with hunger 48 h, the total active and  activity of residualrate had dropped significantly。In the same Cr (VI) concentration dosing groups and levels of hunger residual activity is consistent, that is, the longer the time of hunger, the lower the amount of residual activity。This trend means that compared to Cr (VI) concentration, degree of hunger is anammox bacteria are more important factors。

毕业论文关键词：厌氧氨氧；重金属；抑制作用

Keyword:Anaerobic ammonia oxidation ；heavy metal ；inhibition