摘要：健康、医疗问题一直是世界在努力发展的领域。虽然全世界的整体医疗水平在迅速提高，但许多治疗关键在于前期诊断。光学显微镜在医疗诊断、分析检测等领域提供基础的检测手段，然而传统的光学显微镜笨重，造价昂贵，操作复杂，很难普及，不利于医疗事业的发展。在这里我们设计一种便携式光学显微镜，既能获得较优的成像质量，而且体积小，价格低廉。它由廉价的发光二极管、微型光学透镜、CCD芯片等主要光学器件组装成，在很小的空间内可搭建成。经过实验探究，我们在10cm的长度内搭建好显微镜，可实现3m微球的清晰成像，进一步地，我们通过便携式光学显微镜，对人体红细胞、分辨率测试版成像，其结果与配备了EM-CCD的Olympus IX71光学显微镜对比，该便携式光学显微镜的成像性能尚有差距，但基本满足成像需要。我们相信这种光学显微镜可在未来有较好的应用前景。77636

毕业论文关键词：光流控、无透镜成像、即时诊断

A Preliminary Study On Optofluidics Imaging and Its Application to Point-of-care Diagnostics

Abstract: The world has been always developing healthcare and heath。 The overall medical standards around the world is increasing rapidly, but the key to healthcare lies in early diagnosis。 Optical microscopes provide the basis for means of testing in medical diagnosis, analysis and detection, and other fields, but the traditional optical microscopes are bulky, expensive and complicated to operate, difficult to spread simultaneously, which is not conducive to the development of health care。 Here we designed a portable optical microscope, both to obtain optimum image quality, and small size, low price。 It is assembled from the inexpensive light-emitting diodes, micro-optical lens, CCD chips and other optical devices in a small space。 Through the experiments, we build a microscope of good quality within 10cm in length, which can achieve the clear imaging of microspheres of 3m, Furthermore, we obtained the clear microscopic imaging of human red blood cells, beta-resolution imaging by the portable optical microscope。 The results of which are contrasted with the Olympus IX71 optical microscope equipped with EM-CCD 。 We find that the portable optical microscope imaging performances are not as good as commercially available precision optical microscope, but basically meet the imaging needs。 We still believe that this kind of portable optical microscope has a promising future prospects。

 Keywords: optofluidics; lensfree imaging; point-of -care (POC) diagnostics

目录

摘要： 3

前沿 4

实验部分 4

1。实验原理 5

2。实验装置 5

3。实验步骤 6

3。1筛选透镜 6

3。2优化光源 7

3。3采集数据 8

4。实验数据 8

实验结果与讨论 11

结论 12

参考文献 12

便携式光学显微镜的研制及其性能初步研究

前沿 光学成像技术在发展即时检测医疗器件方面意义非凡，然而传统显微镜体积大、成本高等缺点限制了其在野外等苛刻环境下的使用。微型光学显微镜是光流控学的研究内容之一，在环境监测、安全、卫生保健等方面具有广阔的应用前景。目前大多数发展中国家以及贫困落后地区仍是疟疾、艾滋病和肺结核等传染病的高发区，然而这类地区的医疗器械始终极度缺乏[1]。随着社会的发展和人道组织的推进，医疗救助领域对远程医疗和即时检测的需求日益增加，急需易用、耐久、廉价的便携式显微镜。因此人们对价格低廉、使用方便的医疗器械的诉求日益增加[2-14]。随着科学技术向小尺度与低维空间的推进以及显微技术的发展，人们对高成像质量的便携式微型显微装置的需求越来越强烈[15-52]。论文网