摘要：薄层色谱法是将合适的固定相涂在洗净的载玻片上，形成一均匀平滑完整的层面，然后点样和展开后，比较各物质的Rf值，从而对药物进行鉴别，对杂质进行检查或者对物质含量进行分析测定。本课题首先寻找合适浓度的硅胶和粘合剂，使铺出的板厚度适宜，且不易破损。通过大量练习，学习并掌握铺板方法，能够铺出光滑平整，厚薄适宜的硅胶板，通过实验得到1g硅胶与3至5毫升浓度为1%的 CMC-Na溶液混合铺出来的板效果最好。学会了铺板后，研究不同硅胶板对偶氮苯分离情况的影响，从而选择合适的硅胶板进行后续展开剂研究的实验。在偶氮苯展开剂配方研究的实验中，乙酸乙酯：环己烷=1:6分离效果最好，环己烷：苯=3:2分离效果最好。在圆珠笔芯油展开剂的选择研究中，通过查阅文献，得知芯油要用乙醇试剂溶解，展开剂选择正丁醇，乙醇和水的混合液，且最佳配比为正丁醇：乙醇：水=9:1:1，实验发现圆珠笔芯油最多能分离出5种颜色，由下向上依次为蓝，浅蓝，灰，黄，浅黄。79885

毕业论文关键词：薄层色谱法；展开剂；硅胶；偶氮苯；圆珠笔芯油

Study on the Selection of Expanding Agent in Thin Layer Chromatography

Abstract: Thin layer chromatography is the appropriate stationary phase coated on the cleaning of the slide to form a uniform smooth and complete layer, and then point and expand, compare the Rf value of each substance, so the drug identification, the impurity Check or analyze the material content。 This topic first find the appropriate concentration of silica gel and adhesive, so that the thickness of the plate out of the appropriate, and not easy to break。 Through a large number of exercises, learning and mastering the plating method, can be spread out smooth, thick and appropriate silica gel plate, by experiment to get 1g silica gel and 3 to 5 ml concentration of 1% CMC-Na solution mixed with the best plate。 After studying the plate, the effect of different silica gel plates on the separation of azobenzene was studied, and the suitable silica gel plate was selected for the experiment。 In the experiment of azobenzene developing agent formulation, ethyl acetate: cyclohexane = 1: 6 had the best separation effect, and cyclohexane: benzene = 3: 2 had the best separation effect。 In the selection of ball-point pen oil development agent, through the literature, that the core oil to use ethanol reagent dissolved, the agent selection of n-butanol, ethanol and water mixture, and the best ratio of n-butanol: ethanol : Water = 9: 1: 1, the experiment found that ball-point pen oil can be separated up to five colors, from the bottom up in order to blue, light blue, gray, yellow, light yellow。

Keywords: Thin layer chromatography; developing agent; silica gel; Ballpoint pen oil 

目录

1 绪论 1

1。1 开题依据 2

1。2 文献综述 2

1。2。1人参健脾片的薄层色谱鉴别 2

1。2。2人参总皂苷含量的薄层色谱应用 2

1。2。3薄层色谱法鉴别红花与复方中的西红花 2

1。2。4研究石油的最佳展开剂 3

1。2。5罗沙替丁醋酸酯合成的薄层色谱展开剂的选择 3

1。2。6红花的薄层色谱鉴别中药材 3

1。2。7叶片提取物抗氧化活性及其薄层色谱的初步研究 3

1。2。8薄层色谱法测定海椒面中苏丹红 4

1。2。9薄层色谱法分离齐墩果酸和熊果酸