黑曲霉液体发酵产蛋白酶工艺条件研究
Study of Liquid Fermentation Conditions of Aspergillus niger to Produce Protrase
Abstract: In this paper, Aspergillus niger as bacteria, protease activity as an index, through the single factor experiment, the effects of different carbon and carbon source concentration respectively, nitrogen sources and different combination ratio, carbon nitrogen ratio, effect of different inorganic salts and inorganic salt concentration on the fermentation of Aspergillus niger Producing protease, defined mediumthe optimal enzyme production of Aspergillus niger, on this basis, through orthogonal experiments, the effects of fermentation conditions on enzyme production of Aspergillus niger fermentation. The results showed that adding 6.5% sucrose, nitrogen source, ammonium chloride compound 3% and peptone 3%, combined with the medium proportion 0.55% sodium chloride, Aspergillus niger protease activity was the highest; the optimum fermentation conditions were: 28 ℃, pH6.6, the volume of liquid is 60mL/250mL. In the 150r/min table speed, shake flask culture of 4d ,measured the maximum protease activity was 2345.72U/mL
KeyWords: Aspergillus niger; Protrase; Fermentation Conditions; Enzyme activity
目录
1.绪论 3
1.1蛋白酶的性质和应用 3
1.2黑曲霉的性质和应用 3
1.3液体发酵 3
1.4本课题的研究目的和意义 3
2. 实验材料及实验方法 3
2.1 使用的材料试剂、仪器设备 3
2.2 拟采取的方法 3
2.2.1 筛选高产蛋白酶的菌株 3
2.2.2 不同碳源种类及浓度对黑曲霉产酶酶活力的影响 3
2.2.3不同氮源种类及比例对黑曲霉产酶酶活力的影响 3
2.2.4 不同C／N值对黑曲霉产酶酶活力的影响 3
2.2.5不同无机盐种类及浓度对黑曲霉产酶酶活力的影响 3
2.2.6初始pH值的确定 3
2.2.7发酵温度的确定 3
2.2.9发酵条件优化 3
2.2.10酶活力的测定 3
2.2.11生长曲线的测定 3
3．实验结果与讨论 3
3.1 酪氨酸标准曲线的测定 3
3.2 高产蛋白酶的筛选结果 3
3.3 生长曲线的确定 3
3.4菌株发酵产蛋白酶最佳碳源及浓度的确定 3
3.5菌株发酵产蛋白酶最佳氮源及比例确定 3
3.5.1无机氮源的分析比较 3
3.5.2有机氮源的分析比较 3
3.5.3复合氮源的比例对于酶活的影响 3
3.6菌株发酵产蛋白酶不同C/N比例的确定 3
3.7菌株发酵产蛋白酶最佳无机盐种类及浓度的确定 3
3.8发酵条件单因素实验 3
3.8.1 不同pH对于黑曲霉产酶酶活的影响 3
3.8.2不同装液量对于黑曲霉产酶酶活的影响 3
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