甘草多糖提取工艺条件研究
毕业论文关键词：甘草；甘草多糖；分光光度法；提取；离心。
Study of Extraction Technical Conditions of Angelica Polysaccharide
ABSTRACT：The radix and rhizoma of 1icorice are the most common and important Chinese traditional medicine．Legume licorice roots and rhizomes are the most common, the most important traditional Chinese medicine. Leguminous plant licorice root and rhizome is the most commonly used, one of the most important Chinese traditional medicine.Licorice contains triterpene, flavonoids and polysaccharide content, this thesis mainly by the method of single factor and orthogonal method to extract the polysaccharide of licorice, single factor experiment, the best technological conditions for extraction solvent for distilled water, extracting temperature is 80 ℃, liquid-solid ratio of solvent and licorice is 30, leaching time is 3 hours, extraction times for 3 times, the extraction of ultrasonic power for 45 KHZ factors selected the best factors to carry out the experiment.Through orthogonal experiment 3 h in the leaching time, leaching temperature 80 ℃, liquid-solid ratio (mL/g) spake, extraction times 3 times.Through the experiments the suction filter in the coarse polysaccharide is sticky, after drying was yellow gray jelly.
Key words：Licorice， GPS， Spectrophotometry， Extraction，centrifuge.
目录
1 绪论 1
1.1 国内外研究现状概述 1
1.2 甘草在食品和药品中的应用及其性质、特征 1
1.3 甘草的化学成分及特征 2
1.3.1 甘草的化学成分 2
1.3.2 甘草的特征 3
1.4 多糖及甘草多糖研究进展和提取方法 4
1.4.1 多糖及甘草多糖的研究概况 4
1.4.2 多糖的提取方法 4
1.5 本课题的研究目的和意义 6
2 实验材料与方法 7
2.1 实验材料 7
2.1.1 试剂 7
2.1.2 实验仪器 7
2.2 实验方法 7
2.2.1 甘草粗多糖的提取工艺流程的确定 7
2.2.2 葡萄糖对照品贮备液的配制 8
2.2.4 苯酚试剂的配制 8
2.2.5 检测波长的确定 8
2.2.6 测定多糖试剂的选择 8
2.2.7 反应溶剂量的选择 8
2.2.8 苯酚-硫酸法反应温度及放置时间的选择 9
2.3 标准曲线的制作 9
3 结果与分析 10
3.1 苯酚-硫酸法反应温度及放置时间的确定 10
3.2 葡萄糖的标准曲线 10
3.3 溶剂的种类对甘草多糖收率的影响 11
3.4 温度对甘草多糖收率的影响 12
3.5 液固比对甘草多糖收率的影响 12
3.6 浸提时间长短对甘草多糖收率的影响 13
3.7 提取次数对甘草多糖收率的影响 14
3.8 超声功率对甘草多糖收率的影响 14
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