多糖合成途径中功能基因的鉴定及其在粘细菌发育中的研究(4)
pMD19-T- 5333 pMD19-T derivative carrying MXAN_5333 This study
pMD19-T- 6908 pMD19-T derivative carrying MXAN_6908 This study
pBJ113 Derived from PUC118 with galK and Kanr Yang Zhaoming and Mao Xiaohua
pBJ113-2921 pBJ113 derivative carrying MXAN_2921 This study
pBJ113-3506 pBJ113 derivative carrying MXAN_3506 This study
pBJ113-4613 pBJ113 derivative carrying MXAN_4613 This study
pBJ113-5333 pBJ113 derivative carrying MXAN_5333 This study
pBJ113-6908 pBJ113 derivative carrying MXAN_6908 This study
1．2 引物
表2-1-2实验所用引物
Table 2-1-2 Oligonucleotide primers used in the experiment
引物 序列 (5'-3') 设计目的
2921F CCCAAGCTTGTCCTTGATGCCCTCTGCG Forward primer for MXAN_2921 homologous fragment
2921R CCGGAATTCAAGCCTGCTTCCCATTCGTC Reverse primer for MXAN_2921 homologous fragment
3506F CCCAAGCTTGAAGCGGGTAGCGGTCCT Forward primer for MXAN_3506 homologous fragment
3506R CCGGAATTCGGAACCACGCAATGGTCTC Reverse primer for MXAN_3506 homologous fragment
4613F CCCAAGCTTGGTGGCTGTGGCTTCATCG Forward primer for MXAN_4613 homologous fragment
4613R CCGGAATTCCGCGTTCGGTACTGCGTTTC Reverse primer for MXAN_4613 homologous fragment
5333F CCCAAGCTTGGCATCGTCTTCCACAACATCA Forward primer for MXAN_5333 homologous fragment
5333R CCGGAATTCTTCACGCAGCCGCTCGTT Reverse primer for MXAN_5333 homologous fragment
6908F CCCAAGCTTCCGAGCGAGAACTGTGGGAG Forward primer for MXAN_6908 homologous fragment
6908R CCGGAATTCTGGAGCCCTTGTTGCCGTA Reverse primer for MXAN_6908 homologous fragment
2921tF TGCCCTGGTCCACGATTG Forward primer for identifying
△MXAN_2921
2921tR TCTGGCAACACCTCCCCC Reverse primer for identifying
△MXAN_2921
3506tF ACGTTCGACGAGCTGTGAGAG Forward primer for identifying
△MXAN_3506
3506tR GACCTTCACGCAACCTCCC Reverse primer for identifying
△MXAN_3506
4613tF TGGAGCAAGGAACACACGC Forward primer for identifying
△MXAN_4613
4613tR GTGGACCCCTTCGATACGC Reverse primer for identifying
△MXAN_4613
5333tF CCCGGTGACCTGGTCCTCT Forward primer for identifying
△MXAN_5333
5333tR TGGGCGCGTAGAGGTAGGT Reverse primer for identifying
△MXAN_5333
6908tF GCGAAAACTGCCAGGAGGA Forward primer for identifying
△MXAN_6908
6908tR CGATGAAGGTGGCGAAGAA Reverse primer for identifying
△MXAN_6908
2921exF TCCCCGGCATCCACGCGCGCTA Forward primer for testing the expression of MXAN_2921
2921exR GTTCCGAAGCCTGGATGCGCA Reverse primer for testing the expression of MXAN_2921
3506exF GGCCTCGCCGGCCTCGCCCA Forward primer for testing the expression of MXAN_ 3506
3506exR GTCTACGACGAGGCCAAGCG Reverse primer for testing the expression of MXAN_3506
4613exF CGCCGGCCCGGCCCTTCTCCA Forward primer for testing the expression of MXAN_4613
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