高温胁迫下外源亚精胺对黄瓜幼苗生长及叶绿体超微结构的影响
毕业论文关键词：黄瓜；高温胁迫；外源亚精胺；叶绿素；叶绿体超微结构
Effects of Exogenous Spermidine on growth and chloroplast ultrastructure of Cucumber Seedlings under high temperature stress
Abstract：This paper takes the Cucumber Variety 'high temperature sensitive Jinchun 2' as experimental material, the chlorophyll content of cucumber seedlings were measured. The changes of growth index and chloroplast ultrastructure, studied the effect of high temperature stress of Exogenous Spermidine on growth and chloroplast ultrastructure of cucumber seedlings. The results showed that: (1) in normal conditions, no significant effect on the growth indexes of cucumber seedlings exogenous spermidine on seedling and chloroplast structure. (2) under high temperature stress, the chlorophyll content of cucumber seedlings, the fresh weight and dry weight than normal, decreased significantly; under high temperature stress, the starch grains in the chloroplast increased significantly, the grana lamellae and squeezed out of order, and in the film layer also appeared on the osmiophilic particles, the chloroplast structure deformation. (3) under high temperature stress exogenous spermidine, can effectively alleviate the high temperature on the growth of Cucumber Seedlings The inhibition also increased the chlorophyll content of cucumber seedlings; exogenous spermidine can reduce the chloroplast accumulation of starch, ease the extrusion of grana lamellae, which are orderly arranged, also reduced the number of osmiophilic particles. The results show that exogenous spermidine can effectively change the stability of high temperature stress cucumber chloroplast structure, increase the content of chlorophyll.
Key words: cucumber；high temperature stress；exogenous spermidine；chlorophyll；chloroplast ultrastructure
目 录
摘要： 3
关键词： 3
Abstract： 4
Key words: 4
1 材料与方法 5
1．1 材料与处理 5
1．2 测定方法 5
1．2．1 叶绿素含量及生长指标的测定 5
1．2．2 叶绿体超微结构的观察 5
1．3 数据分析 5
2 结果与分析 6
2．1 高温胁迫下外源亚精胺对黄瓜幼苗生长及叶绿素含量的影响 6
表2-1外源Spd对高温胁迫下黄瓜幼苗生长和叶绿素含量的影响 6
图2-1 外源Spd对高温胁迫下黄瓜幼苗叶片的影响 7
2．2 高温胁迫下外源亚精胺对黄瓜幼苗叶绿体超微结构的影响 7
图2-2 外源Spd对高温胁迫下黄瓜幼苗叶绿体超微结构的影响 8