摘要：本研究通过对网纹草进行0。1%、0。2%、0。4%、0。6% 盐浓度的胁迫处理，测定叶片过氧化物酶（POD）活性、丙二醛（MDA）含量、蛋白质含量以及叶绿素含量，来探究盐胁迫对网纹草叶片生理功能的影响。结果表明，在胁迫初期，0。1%-0。6% 的盐胁迫使网纹草叶片过氧化物酶（POD）活性分别比对照下降了76%、75%、114%、135%；网纹草叶片丙二醛（MDA）含量明显增加，与对照相比分别增加了3%、3%、6%、10%；网纹草叶片蛋白质含量对照比，依次下降10%、28%、19%、42%；网纹草叶片叶绿素含量均有所下降，与空白对照相比依次下降1。7%、0。4%、1%、0。6%。在盐胁迫第三周，同一盐浓度下与胁迫初期比，叶片POD活性依次上升94%、60%、126%、165%，基本恢复至正常水平，而叶片MDA含量依旧逐渐上升，但上升速度明显减缓，整体都低于空白对照下的正常水平。叶片蛋白叶绿素含量则仍呈现明显的下降趋势，但下降速度也开始减缓，与对照的正常水平值相差不大。82103

关键词：盐胁迫 抗氧化酶 网纹草 过氧化物酶翻

译成功

Effects of salt stress on activities of antioxidant enzymes in netted grass leaves

Abstract:In this study, we studied the activities of chlorophyll (POD), malondialdehyde (MDA), protein content and chlorophyll content in leaves of reticulata by 0。1%, 0。2%, 0。4% and 0。6% salt concentration。 To investigate the effects of salt stress on the physiological function of the leaves of Anabaena indica。 The results showed that the activity of peroxidase (POD) in leaves of Anabaena platyphylla decreased by 76%, 75%, 114% and 135%, respectively, at the initial stage of stress, 0。1% -0。6% (P <0。05), and the content of MDA was increased by 3%, 3%, 6% and 10% respectively compared with the control。 The protein content of reticulata leaves decreased by 10%, 28%, 19% and 42% And the chlorophyll content of the leaves decreased by 1。7%, 0。4%, 1% and 0。6%, respectively。 In the third week of salt stress, the POD activity increased by 94%, 60%, 126% and 165% in the same salt concentration compared with the initial stress, and the content of MDA in leaves decreased gradually, but the increase rate Significantly slowed down, the overall lower than the normal level of the blank control。 Leaf protein chlorophyll content still showed a significant downward trend, but the rate of decline began to slow down, and the control of the normal level of little difference。

Keywords: salt stress, antioxidant enzymes, netted grass, peroxidase

目录

1绪论 1

   1。1引言 1

   1。2本课题的研究意义 2

2盐胁迫对植物的影响 3

   2。1盐分对植物的胁迫机理 3

   2。2研究现状 4

   2。3发展趋势 6

3实验材料与研究方法 7

   3。1网纹草简介 7                                       

   3。2实验设计及方法 9

      3。2。1实验设计 9

      3。2。2实验方法 10

4结果与分析 12

   4。1 盐胁迫对网纹草叶片过氧化物酶活性的影响 12