外源葡萄糖对盐胁迫下黄瓜幼苗生长和抗氧化代谢的影响
毕业论文关键词：盐胁迫；黄瓜；葡萄糖；抗氧化代谢；基因表达
Effects of exogenous glucose on growth and antioxidant metabolism of cucumber seedlings under salt stress
Abstract：Effects of exogenous glucose (100mM) on the growth and antioxidant metabolism of cucumber seedlings under salt stress (75mM) were studied using cucumber cultivar ‘Jinyou No4’ as experimental material and nutrient solution cultivation. The results showed that the normal growth of cucumber seedlings under salt stress was inhibited, while exogenous glucose on leaf surface could alleviate the growth inhibition induced by salt stress. Salt stress significantly increased malondialdehyde (MDA) content of cucumber leaves and the production rate of superoxide anion (O2∙-), decreased superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) activity. Under salt stress, the application of glucose significantly improve cucumber leaf SOD, POD and APX activity, reduced O2∙- production rate and MDA content. The results showed that exogenous glucose by regulating the expression of cucumber seedlings under salt stress of antioxidase encoding genes, improving the antioxidase activity, reducing oxidative damage, promoting seedling growth, so as to improve the salt tolerance of cucumber plants.
Key words: salt stress; cucumber; glucose; antioxidant metabolism; gene expression
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1材料与方法 1
1. 1幼苗培育及处理 1
1.1.1幼苗培育 1
1.1.2试验处理 1
1.2 测定方法 1
1.2.1生长指标测定 1
1.2.2抗氧化酶活性分析 1
1.2.3 MDA含量与O2∙-产生速率测定 1
1.2.4 黄瓜幼苗叶片总RNA的提取 1
1.2.5 黄瓜叶片RNA反转录 1
1.2.6 引物设计和PCR反应体系 1
1.3数据分析 1
2 结果与分析 1
2.1 外源葡萄糖对盐胁迫下黄瓜幼苗生长的影响 1
2.2 外源葡萄糖对盐胁迫下黄瓜幼苗叶片抗氧化酶活性的影响 1
2.3 外源葡萄糖对盐胁迫下黄瓜幼苗叶片O2∙-产生速率和MDA含量的影响 1
2.4 外源葡萄糖对盐胁迫下黄瓜幼苗叶片中的基因表达的影响 1
3 讨论 6
致谢 1
参考文献 1
图1 外源葡萄糖对盐胁迫下黄瓜幼苗叶片SOD、POD和APX活性的影响 5
图2 外源Glu对盐胁迫下黄瓜幼苗 O2∙-产生速率和 MDA含量的影响 5
图3 外源葡萄糖对盐胁迫下黄瓜叶片抗氧化酶编码基因表达的影响 6