外源钙对梨石细胞形成及不同形态POD的影响研究
毕业论文关键词：梨；石细胞；木质素；POD
Effects of Exogenous Calcium on the Formation of Pear Stone Cells and the Different Forms of POD
Abstract：Choosing ‘Dangshansuli’ as experimental materials, the treatment group was sprayed with 0.25%, 0.5%, 0.75% exogenous calcium. The control group was sprayed with clean water. The results showed that spraying 0.25%, 0.5% calcium chloride could reduce the content of stone cell, lignin content and the activity of cytoplasmic peroxide（POD I） and cell wall bound peroxidase(POD II); spraying 0.75% calcium chloride could increase the content of stone cell, lignin content and the activity of peroxide(POD); the contents of stone cells, lignin and POD II increased first and then decreased during fruit development. Spraying 0.5% calcium chloride on the leaves is the best way to reduce stone cells; In contrast to POD I, there was a great correlation between the POD II and stone cell formation.
Key words: pear（pyrus）; stone cell; lignin; POD
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法1
1.1 材料 2
1.2 方法 2
1.2.1 冷冻法测石细胞含量2
1.2.2 Klason法测木质素含量2
1.2.3 石蜡切片观察石细胞团2
1.2.4 GPOD活性测定2
1.2.5 SPOD活性测定2
1.2.6 细胞质及细胞壁结合POD（POD I和POD II)的提取与活性测定2
2 结果与分析2
2.1石细胞团石蜡切片的显微观察及钙处理下石细胞、木质素含量的变化3
2.1.1梨果实发育过程中石细胞直径变化和石细胞团石蜡切片的显微观察3
2.1.2梨果实发育过程中不同钙浓度处理下石细胞含量的变化3
2.1.3梨果实发育过程中不同钙浓度处理下木质素含量的变化4
2.2 梨果实发育过程中钙处理下不同形态POD活性的变化5
2.2.1 愈创木酚过氧化物酶含量的变化5
2.2.2丁香醛连氮过氧化物酶含量的变化7
3 讨论 9
3.1外源钙对石细胞、木质素含量的影响 9
3.2不同形态POD活性的研究和外源钙对不同形态POD活性的影响 10
3.3改善梨品质的建议 11
致谢11
参考文献11
图1‘砀山酥梨’果实石细胞团直径的变化3
图2‘砀山酥梨’果实发育期间石细胞团的大小变化3
图3‘砀山酥梨’果实石细胞含量变化4
图4 成熟梨果实中石细胞含量4
图5‘砀山酥梨’果实木质素含量变化5
图6 成熟梨果实中木质素含量5
图7果实发育过程中愈创木酚细胞质过氧化物酶（GPOD I）活性变化6
图8果实发育过程中愈创木酚细胞壁结合过氧化物酶（GPOD II）活性变化6
图9 果实发育过程中愈创木酚过氧化物酶（GPOD）总活性7
图10果实发育过程中丁香醛连氮细胞质过氧化物酶（SPOD I）活性变化8
图11 果实发育过程中丁香醛连氮细胞壁结合过氧化物酶（SPOD II）活性变化8
图12 果实发育过程中丁香醛连氮过氧化物酶（SPOD）总活性9