亚精胺对番茄幼苗高温胁迫伤害的缓解作用
毕业论文关键词：番茄；高温胁迫；亚精胺；徒长
The Effectof Spermidine Alleviating the High Temperature Stress Injury onTomato Seedlings
Abstract:This experiment use the tomato cultivar 'Puhong 968' as experimental material, the cultivation mode is substrate culture,and we set up four treatments: spraying distilled water on the leaves at room temperature, spraying spermidine (Spd) on the leaves at room temperature, spraying distilled water on the leaves at high temperature, spraying Spd at high temperature, study the effects of exogenous spermidine under the high temperature stress on tomato seedlings. The results showed that high temperature stress reduced the dry weight of the overground and the underground parts of tomato seedlings. Under the stress condition, the dry weight of the underground increased significantly compared with the seedlings under high temperature stress, and increased by 5.79% when treated with Spd. And the dry weight of the overground recovered to the level as normal. Under the high temperature stress, the content of carotenoid did not change significantly and the content of chlorophyll a, chlorophyll b declined slightly. The content of chlorophyll a, chlorophyll b and carotenoid increased significantly when treated with Spd under the high temperature stress, and increased by 31.80%, 30.23% and 32.24% respectively. High temperature stress significantly reduced the tomato leaves net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr), declined by 44.39%, 69.23% 28.34% and 58.13% compared with control respectively. Under high temperature stress, spraying Spd significantly increased the content of Pn, Gs, Tr and Ci compared with the control, respectively increased by 27.83%, 50%, 20.23% and 51.02%. These results indicated that high temperature stress affects the photosynthesis of tomato seedlings, mainly because that it broke the balance between photosynthetic pigment synthesis and degradation, and Spermidine could alleviate the inhibition of high temperature stress on the growth of tomato seedlings by promoting the synthesis of photosynthetic pigments and regulating stomatal factors.
Key words:Tomato; High temperature stress; Spermidine; Spindling
目 录
摘要 1
关键词 1
Abstract 1
Key words 2
引言 2
1 材料与方法 2
1．1 幼苗培育及处理 2