摘要本研究报道转cyp2e1基因调节矮牵牛对甲醛代谢的分子响应分析，即：用15 mg/L的甲醛溶液处理转cyp2e1基因的矮牵牛24 h后。表达谱测序结果显示，甲醛胁迫后，有1613个基因的表达有差异，其中，上调基因975个、下调基因638个。按代谢通路分析，与不同氨基酸、酶代谢相关的有12个，占35%；与光合作用相关的代谢有3个，占9%；与防御与解毒的通路有3个，占9%；其它与激素分泌、辅酶因子、萜类等相关。特别是，苯丙氨酸氨裂解酶、谷胱甘肽-S-转移酶、细胞色素P450、磷酸甘油激酶、热激蛋白等基因均上调表达，而谷氨酰胺合成酶、乙醛脱氢酶、S-腺苷甲硫氨酸脱羧酶、转录因子、叶绿素a-b结合蛋白等基因均下调表达。90014

Abstract

The transgenic cyp2e1 plantlets were cultivated in the culture bottle with 15 g/L formaldehyde stress。 The expression profile was sequenced after 24 h。 The results showed activities of 975 genes were up-regulated and activities of 638 genes down-regulated among 1613 genes。 Based on the KEGG pathway classification, 12 pathways associated with amino acid and enzyme metabolism and 35% of total, 3 pathways associated with photosynthesis and 9% of total, 3 pathways associated with defense and detoxification occupy and 9% of total, and other pathways associated with hormone secretion, coenzyme factor, terpenoids were determinated。 Besides, under formaldehyde stress, the activities of the phenylalanine ammonia lyase, glutathione S-transferas, cytochrome P450, phosphoglycerate kinase, heat shock protein and other various enzymes with functions of defense and detoxification have been up-regulated。 At the same time, activities of glutamine synthetase, acetaldehyde dehydrogenase, s-adenosine methionine decarboxylase, transcription factors, chlorophyll a-b binding protein and other enzymes genes have been down-regulated tendency。

关键词:cyp2e1基因; 矮牵牛; 甲醛代谢; 基因表达

Keywords:cyp2e1 gene; Petunia hybrida; formaldehyde metabolism; gene expression

目  录

目录 3

引言 4

1 材料与方法 4

1。1 材料 4

1。2 RNA提取、源Q于W优E尔A论S文R网wwW.yOueRw.com 原文+QQ75201,8766 纯化及测序文库的构建 5

1。3 测序数据的处理 6

1。4 甲醛处理前后差异表达分析 6

1。5 EST功能注释及分析 7

2结果 7

2。1 测序的数据质量统计结果及Reads质量评估 7

2。2 差异表达分析 9

2。2。1 EST表达量分析 9

2。2。2 EST的差异表达分析 9

2。3 EST功能注释及分析的结果 10

3讨论 13

参考文献 15

致  谢 16

引言来自优Y尔L论W文Q网wWw.YouERw.com 加QQ7520~18766

矮牵牛(Petunia hybrida)是茄科矮牵牛属多年生草本花卉植物，因其具有生长快、周期短、易移栽易成活等优点，既是一种观赏植物，又被作为一种极具有代表性的实验模式生物(Wang M et al, 2015)。

有研究报道，甲醛胁迫导致常春藤、吊兰等生理机制发生变化(陈佳瀛等, 2013)。令狐昱慰等(2011)研究常春藤、驱蚊草、绿萝代谢甲醛的情况，结果显示，经甲醛胁迫后，3种花卉植物体内超氧化物歧化酶(SOD)和过氧化物酶(POD)的含量显著提高，而过氧化氢酶的含量没有显著变化，同时丙二醛和脯氨酸的含量随着甲醛浓度的增加有显著的变化。