土壤微生物及蚯蚓辅助松树修复Cr污染土壤的研究
毕业论文关键词：铬，植物根际促生菌，蚯蚓，外生菌根真菌，植物修复
Soil Microorganism and Earthworm（Eisenia foetida） Assisted Pine(Pinus thunbergii) Remediate Cr Contaminated Soil
Abstract：In order to overcome the problem that the plants used in plant remediation are short, grow slowly, and the accumulation of heavy metals in plants is limited, we add earthworms to the tested soils or inoculate plant rhizosphere bacteria and ectomycorrhiza Fungi to improve the efficiency of phytoremediation. We setup 3×2×4 factorial treatments of Cr level( 0，100 and 400 mg/kg ), earthworm( no earthworm and with earthworm ) and microorganism( non-microorganism, DGS6, Y4-4 and Pisolithus tinctorius ) organized in a completely randomized design. The results showed that the addition of earthworms in 100 mg/kg and 400 mg/kg chromium contaminated soil increased the total amount of chromium in overground pine by 443.10 % and 116.70 %, respectively, and the total amount of chromium in underground pine increased by 53.41 % and 60.84 %, respectively, compared with the no inoculation of earthworms. In the 400 mg/kg chromium contaminated soil, the total amount of chromium in the underground part of the pine inoculation of Y4-4 was 24.86 % higher than that in the control group, and the inoculation of earthworms and Y4-4 had an interaction in increasing the total amount of chromium in the plant. This study provides a basis for the combined application of earthworms and plant growth-promoting rhizobacteria to assist phytoremediation of heavy metal contaminated soil.
Key words: Chrome；plant rhizosphere promoting bacteria；earthworm；ectomycorrhizal fungi； phytoremediation
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法3
1.1 材料 3
1.2 实验设计 3
1.3 土壤有效态Cr的测定4
1.4 植物积累重金属含量的测定4
1.5 数据统计分析4
2 实验结果4
2.1 蚯蚓对日本黑松修复铬污染土壤的影响4
2.2 植物根际促生菌及外生菌根真菌对日本黑松修复铬污染土壤的影响5
2.3 施加蚯蚓及接种植物根际促生菌及外生菌根菌对日本黑松修复铬污染土壤的协同效应6
3 讨论7
致谢7
参考文献8
土壤微生物及蚯蚓辅助松树修复Cr污染土壤的研究
土壤中重金属污染是指由于人类活动将重金属带入到土壤中，致使土壤中重金属含量明显高于背景含量，并可能造成现存的或潜在的土壤质量退化、生态与环境恶化等现象[1]。土壤中的重金属主要来源于废弃的矿产、大气中沉降的重金属、工业废水和生活中排放的污水、污泥肥料和农药等。重金属进入环境初期，危害不明显且难以被察觉，当在土壤中迁移的重金属超出一定值并随食物链向人类传递积累时，才显现出了毒害效应。目前修复土壤重金属污染的方法有物理化学修复和生物修复。生物修复包括植物修复和微生物修复，由于生物修复具有成本低廉、操作简单、修复效果好、不产生二次污染、能够大规模推广应用等优点，已经成为研究修复土壤重金属污染的热点。