水稻耐低氮性状的QTL定位+文献综述
毕业论文关键词：氮元素；普通野生稻；CSSL；QTL 定位。
QTL Mapping of nitrogen utilization related traits in riceAbstract：Nitrogen is a necessary major element during the crop growth and development, the shortage ofnitrogen supply will decrease the crop yield, however, excessive nitrogen will destory the ecologicalenvironment, cause environment pollution, so the reasonable application of nitrogen fertilizer and theapproach for improving the efficiency of nitrogen use is particularly important. In this experiment we usethe Oryza rufipogon Griff. and the Oryza indica. varieties 93-11 for the recurrent parents constructs a totalof 200 CSSLs, by controlling the nitrogen supply, the normal field nitrogen fertilizer supply is 16kg/mu,phosphate fertilizer, potash fertilizer is 4.5kg/mu, as control group. Low nitrogen fields do not applynitrogenous fertilizer as the experiment group, every group repeats two times. By analysising andcomparing the different agronomic traits between two group, the following results were obtained: 1.Thefrequency distribution table of different traits accord with normal distribution approximately. The plantheight of low nitrogen field is positively correlated with the of normal field, and the effective number ofpanicle, and grain width are negatively correlated. 2. using the Inclusive composite interval mappingfor QTL mapping, to find a total of 5 QTL loci, among the loci may have some genes control nitrogen useefficiency, nitrogen absorption, assimilation.Key words: Nitrogen element；OryzarufipogonGriff ；CSSL；QTL Mapping.
目 录
摘要3
关键词3
Abstract…3
Key words3
第一章 引言…3
1.1 氮素在植物体内生长发育的作用…3
1.1.1 植物中的氮源 …3
1.1.2 氮素在植物体内的营养功能 4
1.2 植物氮素的吸收和利用机制…4
1.3 水稻氮素利用相关研究进展…4
1.4 植物数量性状的 QTL 定位研究5
1.5 文章的立论依据和意义…5
第二章 材料和方法5
2. 1 实验材料…5
2.1.1 实验材料来源和种植… 5
2.1.2 材料表型数据的收集 … 5
2.2 实验方法及数据分析方法6
2 . 2 . 1 D N A 的提取… 6
2.2.2 聚合酶链式(PCR)反应… 6
2.2.3 聚丙烯酰胺凝胶电泳检测 7
2 . 2 . 4 银染显色… 7
2. 2 .5 表型数据分析方法 … … 7
2 . 2 . 6 Q T L 分析… 7
第三章 结果与分析8
3.1 群体表型分析…8
3.2 群体 QTL 定位分析…9
第四章 讨论与展望10
4.1 讨论：本研究检测到的 QTL 位点与前人研究结果的比较 …10
4.2 本研究得到的成果展望…10
致谢…11
参考文献11
下一篇：水稻苗期耐盐相关性状关联分析