花烛‘Sonate’叶色突变体和野生型有色体发育和类胡萝卜素积累特征研究
毕业论文关键词：花烛；叶色突变体；类胡萝卜素；有色体；蔗糖和光照处理花烛；
Study on the chromoplast development and characteristics of carotenoid accumulation of Anthurium 'Sonate' leaf color mutant and the wild type
Abstract：The accumulation of carotenoids not only can affect plant leaf color and flower color, protecting the plant tissue from the strong sunlight and improving the growth potential and adaptability of plants, but also improve the nutritional value of horticultural crops and crop products organs. Anthurium is of an important ornamental flower, of which the bract color, size, plant type (for potted-flowers or cut-flowers) and leaf pattern and other target traits have been widely studied, but the characters of Anthurium leaf color mutation is rarely reported. In this study, we analysed the effects of carotenoid accumulation on the leaf color difference based on Anthurium leaf mutant dark green and wild type with the treatment of sucrose and light, preliminarily inquiring into the reason of leaf color difference in dark green and wild type. Our results showed that (1) Under normal growth conditions, carotenoid contents of Anthurium dark green and wild type of young leaves have no significant differences; (2) With the treatment of different sucrose concentration, dark green is more capable of accumulating carotenoids than that in the wild type; (3) In the strong light treatment, the ability of resistance to the decomposition of carotenoids in dark green is stronger as compared with the that in wild type.
Key words: Anthurium; Leaf color mutant; Carotenoid; Chromoplast; Sucrose and Light treatment
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1 材料与方法 3
1.1 花烛突变体和野生型叶片显微结构观察 3
1.1.1 试验材料3
1.1.2 试验方法3
1.2 蔗糖对花烛突变体和野生型类胡萝卜素积累的影响 3
1.2.1 试验材料3
1.2.2 试验方法3
1.3 光照强度对花烛突变体和野生型类胡萝卜素积累的影响 4
1.3.1 试验材料4
1.3.2 试验方法4
2 结果与分析 4
2.1 花烛突变体和野生型叶片显微结构观察 4
2.2 蔗糖对花烛突变体和野生型类胡萝卜素积累的影响 5
2.2.1 未处理花烛突变体和野生型类胡萝卜素的含量测定5
2.2.2 不同蔗糖浓度下花烛突变体和野生型类胡萝卜素含量的分析 5
2.2.3 不同蔗糖浓度下花烛突变体和野生型叶绿素含量的分析7
2.3 光照强度对花烛突变体和野生型类胡萝卜素积累的影响 7
2.3.1 不同光照强度对花烛突变体和野生型叶色的影响 7
2.3.2 正常光照条件下花烛突变体和野生型类胡萝卜素含量测定 9
2.3.3 不同光照强度下花烛突变体和野生型类胡萝卜素含量的分析 10
2.3.4 不同光照强度下花烛突变体和野生型叶绿素含量的分析11