索拉胶寡糖的抗氧化活性研究
关键词 索拉胶寡糖 抗氧化 肝损伤
毕业论文设计说明书外文摘要
Title Antioxidant Activities of Salecan oligosaccharide
Abstract
As the hydrolyzate of a novel β-(1, 3)-D-glucan ---Salecan, Salecan oligosaccharide has the characteristics of uniform molecular weight，as well as easy to separation and purification.This thesis focuses on the antioxidant activity of Salecan oligosaccharide.In vitro experiment showed that salecan oligosaccharide can effectively remove the • OH radicals.In vivo study,C57BL/6J mice were pided into normal group, model group, low dose oligosaccharide group (40 mg/kg), high dose oligosaccharide group (80 mg/kg).Intraperitoneal injection of corresponding reagent once daily for 4 days, 4 hours after the last injection ,mice received ip of 0.5 ml/kg dose of CCl4. Collected blood and liver tests, we found that serum transaminase (ALT and AST) in the group that pretreatment salecan oligosaccharide are significantly lower than the model group,and we can see from the antioxidant gene expression, salecan oligosaccharides can increase their expression. The results show that the SO8 has radical scavenging activity in vitro and in vivo activity of antioxidant injury.
Keywords Salecan oligosaccharide antioxidant liver injury
目 次
1 绪论 1
1.1 寡糖的定义 1
1.2 寡糖的分类 1
1.3 寡糖的制备方法 1
1.4 寡糖的生物学活性 2
1.4.1 抑菌作用 2
1.4.2 调节肠道益生菌 2
1.4.3 抗病毒活性 2
1.4.4 免疫调节作用 3
1.4.5 抗氧化活性 3
2 索拉胶寡糖的制备及体外抗氧化实验 4
2.1 实验材料 4
2.1.1 实验试剂 4
2.1.2 培养基配方 4
2.1.3 实验仪器 5
2.2 实验方法 5
2.2.1 索拉胶寡糖的制备 5
2.2.2 分离纯化索拉胶寡糖 6
2.3 体外抗氧化实验 ——清除羟基自由基的测定 7
2.3.1 原理 7
2.3.2 方法 7
2.4 结果与讨论 8
3 索拉胶寡糖对四氯化碳引起的急性肝损伤的影响 9
3.1 实验材料 9
3.1.1 生物材料 9
3.1.2 实验试剂 9
3.2 实验方法 11
3.2.1 索拉胶寡糖试剂的制备 11
3.2.2 建立CCl4急性肝损伤模型 11
3.2.3 血清转氨酶的测定 11
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