靶分子触发荧光探针制备研究+文献综述
本次毕业设计基于金纳米粒子的荧光粗灭性能，设计合成了金纳米分子信标，利用miRNA-21作为靶分子，触发分子探针发出荧光，实现简单、快捷的癌症诊断。
关键词 金纳米 分子探针 miRNA-21
毕业论文设计说明书外文摘要
Title Studies on the Preparations for the Trigger of a Fluorescent Probe from Target Molecules
Abstract
The molecular probe (molecular beacon, MB) is a fluorescent probe in which oligonucleotide is the main component. It makes use of fluorophore and quencher to carry on the molecular detection with high sensitivity and high specificity, when compared with other detection methods. It has the advantage of simple operations and great applications in the fields of biology and medicine. The small RNA (micro RNA, mi RNA)，which has been found to be only 20-25 BP in recent years, is a class of endogenous non-coding single-stranded RNA. It plays a negative regulatory role in the expression of genes，and has influences on the cell growth, development and apotheosis of biological processes. Since it has been found in Clinic that the mi RNA-21 expression levels have close ties to the clinical of tumor and development, the diagnoses of the expression levels of mi RNA-21 have become a new hot spot for the gene therapy means of cancer.
The graduation project, which was based on the properties of gold participles to rough off the fluorescent，designed and synthesized the gold molecular beacons，using mi RNA-21 as the target molecule to trigger the fluoresces of the gold molecular beacons, achieve the aim of simple and quick diagnosis of cancer.
Keywords gold participles molecular beacons mi RNA-21
目 次
1 引言 1
1.1 mi RNA-21的生物学概述 1
1.1.1 mi RNA-21的位置与表达 1
1.1.2 mi RNA-21的调节机制 1
1.1.3 micro RNA的检测的挑战 2
1.2 分子信标的研究概况 2
1.2.1 分子信标的结构 2
1.2.2 分子信标的工作原理 3
1.2.3 分子信标的参数选择 3
1.2.4 分子信标的发展历程 3
1.3 金纳米分子信标的综述 8
1.3.1 金纳米分子信标的合成 8
1.3.2 金纳米分子信标的应用现状 9
1.4 本次毕设拟开展的任务 9
2 实验过程以及研究方法 11
2.1 实验的准备工作 11
2.1.1 实验试剂 11
2.1.2 实验仪器 11
2.1.3 实验流程 11
2.2 金纳米颗粒的制备 11
2.2.1 金纳米粒子的合成 11
2.2.2 金纳米粒子的表征 12