海洋生物共生真菌活性天然化合物的发现研究
关键词 海洋共生菌 次生代谢产物 炭球菌属 生物活性
毕业论文设计说明书外文摘要
Title Study on the Active Secondary Metabolites of Marine-Derived Fungi
Abstract
Because of the particular extreme conditions the marine-derived fungi live in，we can often find many secondary metabolites， with various novel structure , distinctive functions and various biological activities, from marine-derived fungi, the research of them is significant way of seeking for new drugs．This article has studies on the metabolites from a marine-derived fungus Daldinia eschscholzii， which is a red alga associated fungus and was separated from Indonesian and a marine-derived fungus S33-G7， which is a sea urchin fungus and was separated from Lianyungang，Jiangshu . After large scale culture，fermentation，extraction，concentration of the fungus， a total of 8 metabolites，including 2 new compounds identified for the first time ，were isolated by a combination of chromatographies over columns preloaded with Silica gel， Sephadex LH-20 and ODS， and preparative HPLCs. In accordance with their physico-chemical properties and data of NMR(1H-NMR，13C-NMR) and MS ，their structures have been judged.
Keywords marine-associated fungus secondary metabolite Daldinia sp. bioactivity
目 次
1 引言 1
1.1 海洋真菌的研究进展 1
1.2 海洋真菌次级代谢物的研究前景 2
1.3 研究意义及主要内容 3
2 海洋真菌Daldinia eschscholzii次级代谢产物的分离纯化与结构鉴定 4
2.1 技术路线 4
2.2 材料、仪器与试剂 4
2.3 菌种的发酵方法 4
2.4 次生代谢产物的提取与分离 4
2.5 化合物结构的鉴定 4
2.6 生物活性测定 4
2.7 结果与讨论 4
3 海洋真菌 S33-G7 次级代谢产物的分离纯化与结构鉴定 4
3.1 技术路线 4
3.2 材料、仪器与试剂 4
3.3 发酵菌种的筛选 4
3.4 菌种的扩大培养 4
3.5 次生代谢产物的提取与分离 4
3.6 结果与讨论 4
结 论 4
致 谢 4
参考文献4
附录A 主要缩略词和符号 4
附录B 化合物相关图谱 4
1 引言
目前，各种高发疑难病（如癌症、心血管疾病、骨质疏松症、耐药性病原菌感染、病毒感染性疾病、老年痴呆症、自身免疫性疾病等）严重威胁着人类的健康。与此同时，从陆生植物和土壤微生物中发现全新骨架类型成分的可能性逐步降低，这使得天然药物化学工作面临新的问题：新的药源分子从哪里寻找？