铈对重金属胁迫下小麦种子萌发及幼苗生理特性的影响
毕业论文关键词：小麦；铈；铅胁迫；镉胁迫；生理指标
Effects of Cerium on Seed Germination And Seedling
Physiological Characteristics of Wheat under Heavy Metal Stress
Abstract: Using wheat seed as experimental material to study the effects of cerium on seed germination and seedling physiological characteristics of wheat under heavy metal stress with hydroponics. Results were as follows: Seed germination rate of wheat reduced under Pb stress, Cd stress and compound stress of Pb and Cd; The contents of MDA and proline and peroxidase activity of wheat seedling increased under three kinds of stress, while the content of chlorophyll and soluble protein decreased, and content change of soluble sugar was not obvious. However, by CeCl3 (4mg/L) treatment, seed germination rate went up, the content of chlorophyll, proline, soluble protein and peroxidase activity had more increase, while the content of MDA decreased. The results indicated that cerium could reduce the impact of heavy metal stress on wheat and enhance its resistance, and promote the growth of wheat seeding.
Key Words: Wheat；Cerium；Pb stress；Cd stress；Physiological index
目 录
摘 要 1
引言 1
1. 材料与方法 2
1.1 实验材料 2
1.2 试剂 2
1.3 实验方法 2
1.3.1 小麦种子消毒及实验处理设置 2
1.3.2 小麦种子萌发及幼苗的培养 3
1.3.3 镉、铅胁迫液和氯化铈溶液的配置 3
1.3.4 幼苗处理 3
1.3.5生理指标测定方法 3
2. 结果与分析 4
2.1 不同处理下小麦种子的萌发情况 4
2.2 不同处理下小麦幼苗叶片中叶绿素含量的变化 5
2.3 不同处理下小麦幼苗叶片中丙二醛（MDA）含量的变化 5
2.4 不同处理下小麦幼苗叶片中游离脯氨酸含量的变化 6
2.5 不同处理下小麦幼苗叶片中可溶性蛋白含量的变化 6
2.6 不同处理下小麦幼苗叶片中可溶性糖含量的变化 7
2.7 不同处理下小麦幼苗叶片中过氧化物酶活性的变化 7
3. 结论与讨论 8
3.1 结论 8
3.2 讨论 8
参考文献 10
致谢 12
铈对重金属胁迫下小麦种子萌发及幼苗生理特性的影响
引言
小麦是禾本科植物，起源于亚洲西部，是世界上分布最广的粮食作物，并且在粮食作物中播种面积最多。在中国，种植小麦已有5000多年的历史，小麦在河南、山东、江苏、河北、湖北、安徽等省被大量种植。小麦自古就是滋养人体的重要食物。《本草拾遗》这样描写小麦：小麦面，补虚，厚肠胃，强气力。小麦具有养心安神、益肾、健脾、除热止渴的功能，而且食用未精制的小麦还能缓解更年期综合征。小麦是面粉的原料，也是北方人的主食。由于小麦对人们生活的重要作用，所以研究小麦的抗逆性具有重要的意义。
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