钴对集胞藻6803生长的影响研究
毕业论文关键词：钴；集胞藻6803；生长；光合色素；蛋白质
Effects of cobalt on the growth of Synechocystis sp. PCC6803
Abstract: Cobalt is one of the heavy metal elements; it is an important component of vitamin B12 in vivo. However, in the process of industrial production such as metallurgy, dyestuff, ceramic glaze and so on, cobalt was largely discharged into water body, causing corresponding injure to variety of life in case its concentration is beyond a certain range. Algae and quite a lot of protista underwater is the first victim of various heavy metals including cobalt. Besides, heavy metal could be accumulated and enriched in the food chain, giving rise to great threat to consumers at all levels particular human health. Currently, water pollution is increasingly severe, and these subjects are urgently to be solved, for instance, monitoring the situation of heavy metal pollution in the water, study on the effects and molecular mechanism of heavy metal on the growth of typical aquatic organisms, reducing the content and alleviating the toxic effects of heavy metals by means of physicochemical and biological adsorption. Synechocystis sp. PCC6803 is one of the unicellular limnetic cyanobacteria which possess numerous advantages: small inpiduals, quick response to various stresses, well-adapted to a variety of environments and convenient for genetic manipulation, and these characteristics make Synechocystis one of the model organisms in study of stress responses. In the present research, effect of different concentration of cobalt on the growth of Synechocystis sp. PCC6803 was studied. The results showed that cobalt treatment impacts the growth, photosynthetic pigments contents as well as the protein profile of Synechocystis cells significantly. Specifically, when cells were cultured in the medium containing 5µM Co2+, the growth was retarded and the contents of chlorophyll, carotenoid and protein were reduced almost by half. While, 30µM Co2+ almost totally inhibited cell growth and even to die.
Keywords: Cobalt; Synechocystis sp. PCC6803; Growth; Photosynthetic pigments; Protein
目 录
摘 要 4
引 言 5
1 材料和方法 6
1.1 实验仪器和试剂 7
1.2供试材料及其培养 8
1.2.1藻种培养 8
1.2.2重金属处理 8