牛虻唾液腺免疫抑制肽ImmunoregulinHA对单核巨噬细胞分化和功能影响的初步研究
毕业论文关键词：Immunoregulin HA；骨髓源巨噬细胞；精氨酸酶；iNOS
Primary research on how immunosuppressive peptide from salivary glands of the horsefly, Hybomitra atriperoides affect differentiation and function of mononuclear macrophage
Abstract： Immunoregulin HA is an immunopeptide which has been identified and characterized from salivary glands of the horsefly Hybomitra atriperoides. It is composed of 30 amino acid residues with the sequence of GGVTGVTEFEPVDVSGEDYDSDEMDEDGRA. Former research has proved its effects on cytokines secretions in LPS-induced inflammation. In order to investigate immunoregulin HA’s influence on macrophage, we study the homology of the peptide and do research on Arginase and NO generation of bone marrow derived macrophage(BMDM). The results show that immunoregulin HA can distinctly promote Arginase activity of M2 macrophage and can significantly distinctly promote NO generation of M1 macrophage. It means that immunoregulin HA can promote the synthesis of inducible nitric oxide synthase(iNOS). Meanwhile, immunoregulin HA can distinctly promote the NO generation of M2 macrophage under LPS-induced inflammation. Arginase and NO generation are closely related to the cytotoxic effect against viruses and tumor restraint of macrophage.
Key words: Immunoregulin HA；BMDM；Arginase；iNOS
目 录
摘要2
关键词2
Abstract2
Key words2
引言2
1 材料与方法6
1.1 实验材料与试剂6
1.2 实验方法 7
1.2.1 Immunoregulin HA序列同源性分析7
1.2.2 大鼠骨髓源巨噬细胞的分离和诱导7
1.2.3 免疫抑制肽Immunoregulin HA对大鼠骨髓源巨噬细胞精氨酸酶活的影响7
1.2.4 免疫抑制肽Immunoregulin HA对大鼠骨髓源巨噬细胞产生NO的影响8
2 结果与分析8
2.1 Immunoregulin HA序列同源性分析8
2.1.1 实验结果8
2.1.2 结果分析9
2.2 大鼠骨髓源巨噬细胞的分离和诱导9
2.2.1 实验结果9
2.2.2 结果分析9
2.3 免疫抑制肽Immunoregulin HA对大鼠骨髓源巨噬细胞精氨酸酶活的影响10
2.3.1 实验结果10
2.3.2 结果分析10
2.4 免疫抑制肽Immunoregulin HA对大鼠骨髓源巨噬细胞产生NO的影响11
2.4.1 实验结果11
2.4.2 结果分析12
3 讨论 12
致谢14
参考文献14
附录A 仪器及生产厂家16
附录B 英文缩略词表17
牛虻唾液腺免疫抑制肽Immunoregulin HA对单核巨噬细胞分化和功能影响的初步研究
引言 免疫调节物质指一类具有免疫调节功能的存在于生物体内的物质，可以为短肽、脂质、糖肽等，这种物质一般结构多样，在信号通路中通过传递物质、直接作用于靶位点等方式影响免疫应答从而起到调节免疫效应的作用。这种作用一般可以分为免疫激活和免疫抑制。免疫激活指对免疫抑制的生物体可以激活其免疫应答功能，使得生物体的免疫应答强度更大；免疫抑制指对正常或是过敏的生物体可以抑制其免疫应答功能，使得生物体的免疫应答强度减弱。分属于两种作用的免疫调节物质都能对免疫系统产生影响，但其后果的好坏未能肯定。