草甘膦对两种产毒蓝藻的生理效应研究
毕业论文关键词：草甘膦；铜绿微囊藻；绿色微囊藻；急性毒性；藻毒素
Physiological Effects of Glyphosate on Two Toxic Cyanobacteria
Abstract:As a high efficiency, low toxicity, broad-spectrum and ceiling conduction non selective spraying on the leaf buds after herbicide, glyphosate has become one of the largest pesticide around the world, it’s widely used in agriculture. In the same time, the potential environmental risk and safety hazard caused by the large amount of residue in the environment can be obtained. By inhibit the growth of the two Microcystis by glyphosate, effects on protein content, influence on the content of chlorophyll and effects on Microcystin ,to do reseach about algae acute toxicity caused by the glyphosate. The results show that: the concentration for the growth of Microcystis aeruginosa and Microcystis viridis 0.2 mg / L glyphosate did not significantly inhibited, and 2, 5, 10mg / L glyphosate on these two kinds of algae has a significant growth inhibition. At the same time, through a determination of the protein, chlorophyll a, algal cells and microcystin content to detect glyphosate on Microcystis aeruginosa and Microcystis viridis biological effects, directions for future research of the inquiry, in order to provide a valuable reference for the ecological risk assessment.
KeyWords:Glyphosate；Microcystis aeruginos；Acute toxicity；Microcystin；
目录
1引言（绪论） 1
1.1 除草剂的污染及残留 1
1.1.1除草剂的污染现状 1
1.1.2除草剂残留危害 2
1.2草甘膦除草剂的污染现状 2
1.3 蓝藻水华与水体富营养化 3
1.3.1蓝藻水华与水体富营养化发生的原因及现象 3
1.3.2蓝藻水华与水体富营养化的危害 4
1.4微囊藻毒素 4
1.4.1微囊藻毒素毒性 4
1.4.2微囊藻毒素的产生 5
1.4.3微囊藻毒素污染现状 6
1.5课题研究的意义和目的 7
1.6课题研究的内容 7
2实验条件 8
2.1实验仪器 8
2.2实验材料 8
2.3实验药品 9
草甘膦草甘膦的理化性质和药理毒性 10
3实验方案与方法 11
3.1预实验 11
3.1.1 BG11培养液的配置 11
3.1.2高压蒸气灭菌 11
3.1.3铜绿微囊藻的接种 12
3.1.4铜绿微囊藻的培养 12
3.2实验方案论证 12
3.3实验内容与方法 13
3.3.1草甘膦储备液的配置 13