精恶唑禾草灵的水生毒性研究
毕业论文关键词：精恶唑禾草灵；绿色微囊藻；急性毒性；水生浓度
Aquatic Toxicity Fenoxaprop on Microcystis viridis
Abstract: Fenoxaprop have been used extensively in the world and it’s the larger types of herbicide, which can remain in the environment and bring about potential environmental risks. In the present study, the eco-toxicity of fenoxaprop on aquatic life was investigated. Cyanobacterium Microcystis viridis was selected as a test organism. Growth inhibition, protein content, chlorophyll content and cellular enzymes content in Microcystis viridis exposed to fenoxaprop were measured to explore the acute toxicity of fenoxaprop. The results showed that at the environmental concentrations of 0.17 mg/L, 0.19 mg/L and 0.22 mg/L, fenoxaprop could inhibit the growth of cells in Microcystis viridis, and when Microcystis viridis exposed to environmental concentrations of fenoxaprop, the growth was significantly inhibited. Protein and chlorophyll a content were also measured in the study to explore the biological effects of fenoxaprop on Microcystis viridis. Future research directions have been discussed and aimed to provide valuable reference for ecological safety evaluation of this kind of herbicide.
Key Words: fenoxaprop；Microcystis viridis；acute toxicity；aquatic concentration
目录
1. 引言（绪论） 1
1.1 除草剂的原理 1
1.2 除草剂的选择性 2
1.3 除草剂的残留及危害 4
1.3.1除草剂的土壤残留现状 4
1.3.2除草剂的水体残留现状 4
1.3.3 除草剂的生态风险 5
1.4精恶唑禾草灵的药物性质 5
1.5 课题研究的意义和目的 6
1.6 课题研究的内容 7
2. 实验条件及预实验 7
2.1 实验条件 7
2.1.1 实验仪器 7
2.1.2 实验材料 7
2.1.3 实验药品 8
2.2 实验前期准备 9
2.2.1 BG11培养液的配置 9
2.2.2 高压蒸气灭菌 9
2.2.3 绿色微囊藻的接种 10
2.2.4 绿色微囊藻的培养 10
2.3 实验方案论证 10
3. 实验方案与方法 11
3.1 精恶唑储备液的配置 11
3.2 绿色微囊藻生长曲线 11
3.3 考马斯亮蓝染色法测定蛋白质含量 12
3.3.1 实验原理 12
3.3.2 试剂配制 12
3.3.3 实验方法 13
3.3.4 注意事项 15
3.4 绿色微囊藻中叶绿素测定 14
3.4.1 实验原理 14
3.4.2 实验方法 15
3.4.3 注意事项 15
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