苏淮猪耐粗饲相关肠道微生物特征初步解析
毕业论文关键词纤文分解菌; 耐粗饲; real-time PCR; 苏淮猪
Preliminary analysis of the characteristics of intestinal microflora related to roughage feed in Suhuai pigs
Abstract：Crude fiber plays an important role in pig production, providing energy for pigs, improving animal welfare, promoting animal health and improving reproductive performance of sows. Study on the characteristics of pig roughage tolerance can be used to produce new feed resources for pig production, and to some extent alleviate the contradiction between human and pig for grain and reduce feed cost. The fermentation of fiber degradation in intestinal tract of swine mainly from microorganisms, intestinal microbial degradation of fiber has become a research hotspot and an important aspect in the analysis of local pig crude feed tolerance mechanism. Suhuai pig is our self cultivation of new varieties with resistance of crude properties, for its resistance to intestinal microbial mechanism analysis crude, this experiment compared 7 and 8 months of age in Suhuai pigs NDF, apparent digestibility of ADF, July and August were age NDF digestibility of the highest and lowest 5 pigs, the colon and cecum of cellulolytic bacterium by real-time PCR. The difference of digestibility between high and low fiber digestibility, colon and caecum, albino, rumen coccus and Clostridium difficile was studied. The results showed that: NDF, apparent digestibility of ADF of Suhuai pigs in the age of 8 months were significantly higher than that of that of those in the age of 7 months (P<0.05); Albino Ruminococcus abundance in the colon was significantly higher than that in the cecum (P<0.05). For low digestibility group, Clostridium leptum abundance in the colon was significantly higher than that in the caecal (P<0.01). Diferrences of the abundance and the differences of old albino Ruminococcus Clostridium leptum between the colon and cecum of Suhuai pigs in the age of 8 months were not significant. The abundance of albino Ruminococcus and Clostridium leptum in the content of colon and cecum of Suhuai pig in the age of 7 and 8 months were not significant difference between the high and low fiber digestibility group.
Key words: cellulolytic bacteria; crude feeding; real-time PCR; Su Huai pig
目 录
摘要3
关键词…3
Abstract…3
Key words3
引言…3
1材料与方法…4
1.1试验设计…4
1.2日粮及饲养管理…4
1.3样品采集…4
1.4筛选…5
2肠道纤文分解菌数量测定5
2.1纤文消化率测定…5