摘要：近年来抗生素污染已经引起了广泛的关注。其中磺胺甲恶唑（Sulfamethoxazole，SMZ）污染情况比较普遍，危害比较严重。本文采用正交实验，通过调节不同提取液种类、数量，超声时间和淋洗体积优化了土壤SMZ的提取方法，得到以取乙腈/磷酸盐，10mL进行提取，淋洗体积为9mL，超声时间为15min，提取率达到最高，对0.02mg/LSMZ的回收率为85.77%，其中提取液种类为最主要影响因素。此外，本文探究不同抗生素浓度，SPS浓度，Fe2+浓度对SMZ的降解效果，得到硫酸根高级氧化技术降解土壤SMZ的最适条件如下：抗生素浓度50mg/L，SPS浓度10g/L，Fe2+浓度7g/L，此时，24小时，SMZ的降解率达到100%。SMZ浓度(mg/L)是影响降解率的主要因素。43577

毕业论文关键词：磺胺甲恶唑；高级氧化技术；土壤污染

Research on Degradation of Antibiotics in Soil by Sulfate Free Radical Advanced Oxidation Technology

Abstract： In recent years, antibiotic pollution attracts a wide attention. Sulfamethoxazole (SMZ) pollution is more common, and has led to series of environmental problems. In this paper, orthogonal experiment was used to optimize the extraction method of soil SMZ by adjusting the types, quantity, ultrasonic time and elution volume of different extracts. The recovery rate reached the highest when the volume of acetonitrile/phosphate was 10mL, the volume of elution was 9mL, the ultrasonic time was 15min, the extraction rate reached the highest, the recovery rate of 0.02mg / LSMZ was 85.77%. The extracting solution type was the most important influencing factor. In addition, the optimum conditions for the degradation of soil SMZ by sulfate oxidation were studied. The optimum conditions for the degradation of soil SMZ were 50mg/L, the concentration of SPS was 10g/L, the concentration of Fe2 + was 7g/L, and the important of the factors were as follows: the antibiotic concentration, the concentration of SPS and the concentration of Fe2 +. The 24-hour degradation rate reached 100%, of which SMZ concentration (mg / L) was the main factor affecting the degradation rate.

Key words: Sulfamethoxazole；Advanced Oxidation Technology；soil contamination

目  录

摘要1

关键词1

Abstract1

Key words1

引言（或绪论）1

1 材料与方法3

1.1仪器与与试剂 3

1.1.1 仪器 3

1.1.2试剂3

1.1.3 溶液的配制3

1.2方法3

1.2.1土壤中抗生素提取方法的优化3

1.2.2 SMZ降解条件的优化3

1.2.3 抗生素的高效液相色谱测定条件4

2 结果与分析4

2.1 土壤中抗生素提取方法的优化4

2.2 SMZ降解条件的优化 5

3 讨论  7

3.1土壤中抗生素提取方法的优化 7

3.2 SMZ降解条件的优化 8

致谢8

参考文献8

硫酸根自由基高级氧化技术降解土壤中抗生素的研究 引言   近年来，,抗生素因其的环境污染和生态系统毒性已经引起了国内外的普遍关注。资料表明，在我国许多地区抗生素的污染相当严重，我国不同地区土壤中抗生素残留水平存在差异[1]。据有关报道表明，世界磺胺类药物主要由我国生产，并且产量在持续增长，上世纪90年代中期，我国磺胺类药物产量就己经达到1万t，2003年产量更是超过2万t。李彦文等[2]在珠三角地区4种不同类型菜地土壤中测定磺胺甲恶唑的含量范围为19.52~36.74μg·kg-1。

    根据Baguer、Rooklidge、Yang 和 Kuhn[3-6]等人的研究，环境中的抗生素残留会诱导微生物产生耐药性，改变土壤微生物的多样性，抑制微生物、动物、植物的生长发育以及间接影响土壤肥力和养分循环。此外，抗生素尤其是一些难降解、易吸附的种类可通过食物链传输进入人体。长期食用被抗生素污染的农作物和有机蔬菜会对人体健康产生潜在的危害。根据庄珍珍[7]的研究，人如果长期服用SMZ，或是长期饮用SMZ的污染饮用水，会引起人体内的正常的菌群紊乱，从而表现出抗药性或是过敏症状，该类抗生素在人体内积累到一定水平，更是会损坏肾脏，引起尿和造血系统失调。由此可见，SMZ污染情况比较普遍，危害比较严重，修复的价值比较大。