食性结构驱动的两种国产眼镜蛇蛇毒蛋白组含量的变异
Abstract
The research use the Bufo gargarigans or mice to be the food of Naja atra and Naja kaouthia, and then compare the character of their amount of venom as well as components of venom with different prey. According to the result, Naja atra which consumes same prey shows obvious difference in the dry weight of venom; Without the difference of length, the same kind of cobra’s dry weight of venom are almost the same whatever they have consumed, but the indicator of the amount of venom shows an obvious difference that the group feed with toad is higher than the group feed on mice. Snake venom components under reversed HPLC and SDS-PAGE can obtain larger degree of separation, from the two kinds of cobra venom were identified by five to six protien family. Among them, 3-FTx, PLA2 and SVMP-III are the main components of Naja atra,accouting for 67.17%,23.49% and 8.84% respectively before use toad as their food,and after the experiment,accounting for 67.67%,24.13% and 7.78% respectively; accouting for 68.93%,19.84% and 10.56% respectively before use mice as their food,and after the experiment, accounting for 69.06%,20.17% and 10.15% respectively; 3-FTx, PLA2, CRISP and Vespryn are the main components of the venom of Naja kaouthia,accouting for 45.04%,23.35%, 13.39% and 13.77% respectively before use toad as their food,and after the experiment, accounting for 49.84%,17.40%,12.13% and17.27% respectively;accouting for 50.45%,19.97%,9.25% and 17.47% respectively before use mice as their food, and after the experiment, accounting for 51.42%,17.91% ,10.57% and 17.17% respectively.Chi-square test shows, whatever animals consume in a short term, it won’t change the relative percentage of the components in the venom. Thus we know, the change of food may have influence in the dry weight and fresh weight of the venom, but can not have an insignificant influence in the percentage of the components in venom.Certainly, it may also be the raising cycle is not long enough thus different food cannot form obvious influence on the change of venom in such a short cycle.
毕业论文关键词：食物；眼镜蛇；蛇毒；排毒量；组分含量
Key words: prey; cobra; snake venom; the amount of venom; constituent content
目录 4
引言 5
1 材料与方法 5