摘要本文研究了聚二甲基二烯丙基氯化铵（PDM）对铜绿假单胞菌的杀菌性能。以铜绿假单胞菌为受试物种，配制中等污染程度的模拟水样，进行杀菌实验；采用平皿计数法，以杀菌率为考核指标，研究了PDM系列样品的作用浓度、作用时间和特征黏度（[η]值）对其杀菌性能的影响；采用紫外吸收法，分别检测了PDM作用后菌悬液的蛋白质质量浓度与核酸吸光度的变化，以考察胞内物质泄漏情况；通过SEM观察了PDM作用前后细胞表面形态的变化。结果表明：针对菌含量为105个/mL的模拟水样，PDM([η]: 0.59～3.77)的作用浓度为0.1mg/L时，其24h杀菌率为19.5%～43.4%；作用浓度为1.0mg/L时，其24h杀菌率为97.1%～99.9%。PDM的作用浓度和作用时间对杀菌率的影响规律与特征黏度有关，特征黏度越大，所达到的最大杀菌率越大。PDM可以破坏菌细胞的壁、膜结构，使胞内物质泄漏，最终导致细胞死亡。因此，PDM系列样品对以铜绿假单胞菌为代表的革兰氏阴性菌具有很好的杀灭效果，且PDM特征黏度越大，杀菌性能越强。63769

毕业论文关键词  聚二甲基二烯丙基氯化铵（PDM）  铜绿假单胞菌   杀菌性能   杀菌机理

毕业设计说明书（论文）外文摘要

Title Preliminary Study on Bactericidal Action and Mechanism of Polyquaternary Ammonium Salt PDM against Pseudomonas aeruginosa              

Abstract

In this paper, the bactericidal performance of polydiallyldimethylammonium chloride (PDM) was researched. The bactericidal experiment was carried on by using Pseudomonas aeruginosa as the tested species and preparing the simulated water samples with moderate degree of pollution. Using the bactericidal rate as assessment indicator, the effects of the action concentration, action time and intrinsic viscosity ([η] value) of PDM on the bactericidal performance were researched by plate counting method. The changes of protein concentration and nucleic acid absorbance after PDM acting bacterial suspension were detected by the UV absorption method to investigate the leakage of intracellular substances. The morphological changes of surface before and after PDM acting the cells were observed by SEM. The results showed that the 24h bactericidal rates of PDM([η]: 0.59~3.77) were 19.5%~43.4% when the of action concentration were 0.1mg/L, and that of PDM([η]: 0.59~3.77) were 97.1%～99.9% when the of action concentration were 1.0mg/L for the simulated water sample with bacteria content of 105cells/mL. The effects of action concentration and action time of PDM on the bactericidal rates were related to the intrinsic viscosity. The higher intrinsic viscosity value, the greater maximal bactericidal rate. PDM could damage the structures of cell wall and cell membrane to make the leakage of intracellular substances, and finally, the cell died. Therefore, serial PDM samples had an excellent bactericidal effect for Gram-negative bacteria represented by Pseudomonas aeruginosa, and the higher intrinsic viscosity value, the better bactericidal performance.

Keywords  Polydiallyldimethylammonium chloride (PDM)  Pseudomonas aeruginosa  Bactericidal performance   Bactericidal mechanism

 目  录

1 引言 1

1.1 聚季铵盐杀菌剂杀菌性能研究现状 1

1.2 聚季铵盐PDM杀菌性能研究现状 1

1.2.1 PDM简介 2

1.2.2 PDM的杀生性能 2

1.3 聚季铵盐PDM杀菌机理研究现状 3

1.4 本课题的研究内容和目标 4

2 实验原理