摘要本文以耐甲氧西林金黄色葡萄球菌（MRSA）为实验对象，对实验室的2种药用植物中的提取部位及单体化合物进行了抑制和破坏MRSA生物被膜的研究。具体是在96-孔微量培养板中做天然单体化合物对MRSA生物被膜形成抑制实验，结晶紫染色来定量生物被膜，用酶标仪测OD570nm，以确定天然单体化合物是否抑制MRSA生物被膜形成和计算出抑制率，最后筛选出具有抑制MRSA生物被膜形成的单体化合物。根据抑制率，我们认为，来自杂交鹅掌楸的二氯甲烷部位、甲醇部位、正己烷部位、来自白刺的提取物MH-3、MH-6具有抗MRSA生物被膜形成活性，而MH-1、MH-2、LXP-1、LXP-2、LXP-4是否具有抗MRSA活性或抑制活性还有待进一步确认。63770

毕业论文关键词  鹅掌楸  白刺  细菌  耐甲氧西林金黄色葡萄球菌  细菌生物被膜  非经典抗菌 

毕业设计说明书（论文）外文摘要

Title The investigation of non-classical antibacterial extracts or  molecules from two medicinal plants                                            

Abstract

In this paper, anti-biofilm or biofilm inhibition of some extracts and natural compounds from two medicinal plants were studied with pathogenic bacteria (MRSA). All these screenings were carried out in 96 - well microtiter plates, and crystal violet staining to quantify biofilm, measured at OD570nm with a microplate reader in order to determine whether extracts or natural compounds inhibit  MRSA biofilm formation or not, Finally we found a few extract and natural compounds could inhibit MRSA biofilm formation. According to the inhibitory rate, we suggested that three extracts, dichloromethane part, methanol part, n-hexane part, from Liriodendron bybrid, two extracts, MH-3 and MH-6, from Nitraria Tangutorum had anti-biofilm formation activity of MRSA, while whether MH-1, MH-2, LXP-1, LXP-2, LXP-4 had anti-biofilm formation activities or not are still being confirmed.

Keywords  Liriodendron hybrid  Nitraria tanggurum  Bacteria  Methicillin-resistant staphylococcus aureus    Bacterial biofilm    Non-classical anti-bacteria.

1 引言 1

1.1 细菌生物被膜介绍 2

1.1.1 细菌生物被膜的组成成分 2

1.1.2 细菌生物被膜的结构 2

1.1.3 细菌生物被膜的形成过程 3

1.1.4 细菌生物被膜中细菌的生理状态 4

1.2 细菌的群体感应系统 4

1.3 细菌生物被膜可能的耐药机制介绍 5

1.4 耐甲氧西林金黄色葡萄球菌简介 7

2 实验部分 7

2.1 实验材料和实验仪器 7

2.1.1 实验材料 7

2.1.2 实验试剂 8

2.1.3 实验仪器 8

2.2 实验原理 8

2.3 实验步骤 9

2.4实验结果与讨论 11

2.4.1 实验结果 11

2.4.2 实验讨论 18

结  论 20

致  谢 21

参考文献