摘要本文研究了纯菌株Rhizobium sp.NUJST18降解吡啶的关键影响因素及培养条件，并将NUJST18作为接种物培养好氧颗粒污泥，实现高浓度含吡啶废水的生物强化处理。摇床条件下，纯菌株可在10天内实现浓度2500mg/L的吡啶的完全矿化，降解过程伴随着细菌的生长和氨氮的释放；吡啶初始浓度、氨氮浓度、体系pH和外加碳源浓度对吡啶降解具有显著影响；以NJUST18纯菌株作为接种物，成功培养出可降解吡啶的好氧颗粒污泥，颗粒污泥粒径为0.5-1 mm左右；在好氧颗粒污泥反应器系统中，好氧颗粒污泥可在8小时内实现浓度高达4000mg/L的吡啶的完全降解；相比纯菌株体系，好氧颗粒污泥反应器系统的降解速率及耐受浓度显著提高。本文的研究结果表明， Rhizobium sp.NUJST18对高浓度吡啶废水的处理具有较大的应用潜力。63808

毕业论文关键词  好氧颗粒污泥  吡啶  Rhizobium sp.  生物降解  

毕业设计说明书（论文）外文摘要

Title   The cultivation and performance evaluation of aerobic granular sludge for pyridine biodegradation

Abstract Key factors and incubation conditions of pyridine biodegradation by pure strain named after Rhizobium sp.NJUST18 were investigated in this paper, thereafter, NJUST18 was used as the inoculum for aerobic granular sludge cultivation in order to realize the enhanced biodegradation of wastewater containing high concentration of pyridine. Under the condition of incubator shaker, 2500 mg/L of pyridine can be completely mineralized by the pure strain, degradation process is accompanied by the growth of bacteria and the release of ammonia nitrogen. Initial pyridine concentration, ammonia nitrogen concentration, pH and additional carbon source concentration have significant influences on pyridine biodegradation. Inoculated with pure NJUST18 strain, aerobic granular sludge capable of degrading pyridine was successfully cultivated, with the partical size around 0.5-1 mm. In aerobic granular sludge reactor system, 4000 mg/L of pyridine can be degraded completely by aerobic granular sludge within 8 hours. Compared with the pure strain system, the aerobic granular sludge reactor system was  significantly improved in terms of degradation rate and tolerance towards pyridine. This paper shows that Rhizobium sp. NUJST18 has great application potential for concentrated wastewater containing pyridine.

Keywords   Aerobic granular sludge  Pyridine  Rhizobium sp.  Biodegradation

1  引言 1

1.1  吡啶的简介 1

1.2  含吡啶废水的治理方法 1

1.3  好氧颗粒污泥的研究现状 2

1.4  研究课题的提出和内容 5

2  实验材料与方法 7

2.1  实验药品与实验仪器 7

2.2  实验方法 8

2.3  实验表征方法 11

3  结果与讨论 12

3.1  纯菌株体系 12

3.2  好氧颗粒污泥反应器体系 17

3.3 纯菌株体系与好氧颗粒污泥体系对吡啶降解速率的比较 19

结论 20

致谢 21

参考文献 22

1 引言

1.1 吡啶的简介

吡啶是一系列化学品之父，可以使用它作为颜料和橡胶的制备溶剂，用于制药和爆炸品，用于农业杀虫剂和除草剂[1]。它也可以直接用在酒精的变性，并制作成许多不同的产品。所以研究吡啶的生物降解是为了使这种对人体以及对环境有毒有害的物质，分解成无毒无害的物质排放到环境中去，既保护了环境，也保护了化工医药等行业的发展[2]。