摘  要：土壤酶在土壤能量转化和物质循环中具有重要作用，是土壤肥力的重要指标。土壤脲酶活性与土壤氮素转化密切相关, 可以反映土壤氮素水平，是研究较多的土壤酶之一。江苏省淮阴地区为重要的农业产区，盛产多种农产品。本文采用靛酚蓝比色法，对淮阴地区多种农田土壤脲酶开展了初步的调查研究，对了解该地区土壤特性和科学合理施肥具有一定的指导意义。调查研究结果表明：不同农田土壤脲酶活性存在显著差异，玉米和小麦两种作物土壤脲酶活性相比其他样品脲酶活性要高。68300

毕业论文关 键 词：  农田土壤，土壤脲酶

Abstract: Soil enzymes play an important role in energy conversion in the soil and material cycle and that is one of the important indicators of soil fertility. Soil urease activity is closely related to soil nitrogen transformation and can reflect the soil nitrogen levels. Soil urease is one of the most popular soil enzymes. As the important agricultural regions of Jiangsu, Huaiyin district is rich in a variety of agricultural products.According to the colorimetry of indophenol blue,preliminary investigations and studies of soil urease were carried out in various Huaiyin regions,which has a certain guiding significance to know the soil characteristics of the region and the scientific and reasonable fertilization methods.As is shown by the research results,the  the activity of soil urease from different soils is distinct obviously.And compared to that of other samples ,the activities of soil urease from corn and wheat are higher.

Key word：Farmland soil；soil urease

目  录

1  前言 4

2  材料与方法 4

2.1  土壤概况 4

2.2  土样采集 4

2.3  土样的处理 4

2.4   土壤中水分的测量 4

2.5  土壤pH的测定 4

2.5.1  原理 4

2.5.2  主要仪器 5

2.5.3  试剂配制 5

2.5.4  操作步骤 5

2.6  脲酶的测定 5

2.6.1  实验试剂 5

2.6.2  实验方法 6

2.6.3  实验步骤 6

2.6.4  实验结果的计算 6

2.6.5  氮的标准曲线 7

3.  结果与分析 7

3.1  土壤中水分含量 7

3.2  土壤pH的测定 7

3.3  氮元素的标准曲线 8

3.4  土壤中氮含量 8

3.5  无基质对照 9

3.6  试验样品重量对脲酶活性的影响 9

3.7  土样培养时间对脲酶活性的影响 9

3.8  分析 10

结论 11

参 考 文 献 12

致  谢 13

1  前言