摘要在微生物燃料电池（MFC）的研究中，阳极微生物的多样性对于MFC的性能起着至关重要的影响。本实验针对这个问题以河流底泥（SD）、花园表层土壤（TS）、花园深层土壤（1m深度）（DS）为接种物构建MFC。运行周期为一个月，测量、记录它们的产电情况。电池工作一个月后，提取阳极微生物进行高通量测序。通过对高通量数据的分析研究三种不同接种物的阳极微生物种类和群落结构差异。结果表明，SD与DS样品中微生物组分的种类以及所占比例相似度比较高。其中的优势菌为假单胞杆菌目Pseudomonadales，SD中所占的比例为0.449479%，DS中所占比例为0.33036%，而TS中所占的比例仅有0.118885%。在TS中固氮螺菌属Azospirillum、分支杆菌属Mycobacterium，所占的比例为0.218151%和0.183593%。在阳极生物膜群落结构方面，TS的丰度最高，其次为DS，SD的丰度最低。69924

毕业论文关键词：微生物燃料电池  接种物  阳极生物膜  高通量测序 

Effect of different substrates on the yield of MFC and Study on the persity of anode community structure

Abstract

 In microbial fuel cell (MFC) research, the persity of anode has an important influence on the performance of MFC. This experiment aimed at the problem of the river sediment (SD), garden surface soil (TS), the garden deep soil (1m depth) (DS) as the inoculum to build MFC. Operation period is one month, measuring and recording their power production. One month after the battery work, the extraction of anode microorganisms for high throughput sequencing. Through the analysis of high throughput data, the species and community structure of three different kinds of anode were studied. The results showed that the species and the proportion of SD and DS samples were similar. Among them, the dominant bacteria were Pseudomonadales, the proportion of SD was 0.449479%, the proportion of DS was 0.33036, while the proportion of TS accounted for only 0.118885%. In Azospirillum, Mycobacterium, TS, the proportion of 0.218151% and 0.183593%. In the anode biofilm community structure, the highest abundance of TS, followed by DS, the lowest abundance of SD.

Key Words: Microbial fuel cell  Inoculum  Anode biofilm  Illumina Genome Analyzer IIx

目  录

摘要-Ⅰ

AbstractⅡ

目录-Ⅲ

图清单Ⅳ

表清单Ⅳ

1 绪论-1

2 材料与方法1

2.1 MFC的结构与电极材料-1

2.2 MFC的接种物3

2.3 MFC反应器接种和启动-3

2.4 缓冲溶液、有机底物的配方3

3 主要的检测项目和实验方法-4

3.1 电压数据采集-4

3.2 MFC阳极微生物高通量测6

4 实验结构分析8

4.1 微生物多样性以及OUT丰度程度-8

4.2 稀释性曲线9

4.3 Shannon-Wiener曲线分析-10

4.4 Rank-Abundance曲线分析-11

4.5 主成份分析（PCA）11

4.6 样本聚类树与柱状图分析-12

4.7 群落结构组分图分析13

4.8 OUT分布Venn图分析16

4.9 层次聚类分析17

5 结论-18

参考文献19

致谢21

图清单

图序号 图名称 页码

图1-1 单室空气阴极MFC结构图 2

图1-2 单室MFC阳极结构图 2

图1-3 单室MFC空气阴极结构图 3

图2-1 MFC启动周期折线图 5