摘  要自东海海底分离出能够对木质素进行降解的菌株RC。通过对菌株RC的16S rDNA序列的PCR扩增以及测序，在EzTaxon Server数据库中与有较高同源性的菌株序列进行比对，并使用Mega软件对系统发育树进行构建与分析；通过观察RC菌株的菌体形态、大小，菌落的形态、大小、颜色，观察革兰氏染色情况，再用API20NE标准比色卡进行血清学特性的检测了解菌株RC的生理生化特性；通过测定不同PH、温度、NaCl浓度下菌株RC在海水培养基中生长不同时间段的OD600的值研究RC菌株的生长特性。结果表明：RC菌株为革兰氏阴性菌、杆状、有鞭毛，最适生长温度为35摄氏度、NaCl浓度为22.5克/升、PH值为7.0。在系统发育树中RC菌株与Pseudoalteromonas shioyasakiensis sp. nov.（登录号：A720724）聚为一支，置信度为100%。鉴定为假交替单胞菌属(Pseudoalteromonas) [1] 本文通过对RC菌株的研究希望可以为人工降解木质素提供新的思路与相关帮助。69933

毕业论文关键词：假交替单胞菌  生长特性  生理生化特性  系统发育树  降解木质素

Isolation, Identification and Growth Characteristics of strain RC with Lignin Degrade

Abstract East China Sea seabed RC isolated strains were able to degrade lignin.By 16S rDNA sequences of strain the PCR amplification and sequencing of RC in EzTaxon Server database and have high sequence homology to strains were compared, and the use of software Mega phylogenetic tree construction and analysis;By observing cell morphology, size and morphology of colonies, size, color, Gram staining was observed, and then API20NE standard color cards for testing serological characteristics understand physiological and biochemical characteristics of the strain RC; by measuring different PH, temperature growth characteristics under strain RC growth NaCl concentration OD600 different time periods in seawater medium values of RC strain.The results showed that: RC strains of gram-negative, rod-shaped, flagellated, optimum growth temperature is 35 degrees Celsius, NaCl concentration was 22.5 g/L , PH value of 7.0. In the phylogenetic tree RC strains Pseudoalteromonas shioyasakiensis sp. nov. (accession number: A720724) Clade, confidence level of 100%,so Identified as pseudoalteromonas. Based on the RC strains studies hope to provide new ideas and help artificial degrading lignin.

Key Words: Growth characteristics  physiological and biochemical characteristics  phylogenetic tree  lignin degrade  Pseudoalteromonas

目  录

摘要-Ⅰ

Abstract--Ⅱ

目录--Ⅲ

前言1

1材料与方法--2

1.1 实验的材料-2

1.2 RC菌株的分离纯化--2

1.3 RC菌株的鉴定--3

1.4 RC菌株的生长特性的测定--4

1.5 RC菌株的生理生化特性的测定--4

2 结果--5

2.1木质素降解菌株RC的分离纯化与其形态特征-5

2.2木质素降解菌RC菌株的系统发育分析-5

2.3木质素降解菌株RC的生理生化特性-8

2.4 PH值对RC菌株的生长的影响-10

2.5温度对RC菌株的生长的影响10

2.6盐浓度对RC菌株的生长的影响11

3 讨论-12

致谢-13

参考文献-15

1前言

在造纸工业和木材水解工业中会产生一种名为木质素的副产物，木质素及其衍生物是很难降解的物质。未经处理随意排放含木质素的工业废水会造成严重的环境污染，从而破坏生态平衡[2]。但环境中存在许多能对木质素进行降解的微生物[3]。因此利用微生物处理废水中的木质素这一方法在现实应用具有较为广阔的前景[4]。本实验从东海海水中筛选分离出一株对木质素具有降解能力的菌株RC。通过对菌株RC的生长特性的研究，表明RC菌株最适生长温度为35摄氏度、NaCl浓度为22.5克/升、PH值为7.0[5]。此外也对该菌株进行生理生化特性鉴定以及系统发育树的构建与分析，初步将菌株RC鉴定为假交替单胞菌属的Pseudoalteromonas shioyasakiensis sp. nov.[6]。为今后含木质素工业废水降解的社会现实问题的解决提供理论依据。论文网