摘要本文以家蚕真菌白僵菌作为供试菌种，考察了烷基间苯二酚（ C17:0 ， 5- Heptadecylresorcinol）药液的溶剂类型、料液比等对其抑制活性的影响，结果显示石 油醚:无菌水:乳化剂（3:1:1）为其最佳配制方法；在此条件下，通过微量肉汤稀释法 测定烷基间苯二酚的最小抑菌浓度范围是 20~25 μg/mL，最低杀菌浓度为 22。5 μg/mL； 通过光学显微观察测得，影响菌丝生长的药液半抑制浓度 IC50 为 7。19 μg/mL，而影响 孢子萌发的半抑制浓度 IC50 为 14。23 μg/mL；进一步研究其抑菌机理，将不同浓度烷 基间苯二酚（0，2。5，5，10，20 μg/mL）添加入 PDA 培养基，进而考察同一时期白 僵菌的生长情况，生长 5~7 天，肉眼乃至显微镜观察，颜色上并无差异，均为光滑乳 白色，菌落中央略为乳黄，并且随着一定范围内药液浓度的增高，分生孢子梗减少， 孢子产生量降低，出现分生菌丝增多并无孢子产生的现象；最后，将不同浓度烷基间 苯二酚（0，5，10，15，20，25 μg/mL）预处理白僵菌孢子悬浮液 30 min，，同时对 4 龄家蚕进行体表涂抹致病，计算得出家蚕各浓度（溶剂项，0，5，10，15，20，25 μg/mL）下的的致死率分别为 0，26。00%，43。33%，26。67%，23。33%，20。00%，6。67%。80722

毕业论文关键词：烷基间苯二酚；白僵菌；抑制活性

Abstract Silkworm fungus muscardine was used as tested strains to investigate the effect of ARs liquid solvent type and solid-liquid ratio on the inhibition activity。The result showed that petroleum ether: sterile water: emulsifier (3:1:1) was the best preparation method。 Under these conditions, by broth dilution method testing, the minimum inhibitory concentration range of ARs was 20-25 µg/mL, and the minimal bactericidal concentration of ARs was 22。5

µg; by slide optical microscopy measuring, half inhibitory concentration IC50 of ARs liquid affecting mycelium growth was 7。19 µg, half inhibitory concentration IC50 of ARs liquid affecting spore germination was 14。23 µg; Further investigating its inhibition bacteria mechanism, added different concentration ARs ( 0, 2。5, 5, 10, 20 µg /mL) into PDA medium, then examined the growth situation of muscardine during the same period, growing for 5-7 days, by naked eye and microscope observation, had no differences in color and were smooth white, colony-central was slightly whitish-yellow and increased with ARs concentration increasing within a certain range, conidia of infarction reduced, production of spore reduced, hyphae increased and no spore produced。 Finally, different concentrations ARs (0, 5, 10, 15, 20, 25 µg/mL) preprocessed beauveria bassiana conidia suspensions for 30 min and smudge the same fourth age silkworm surface causing disease。 The mortality rate of silkworm with the different concentration ARs was 0,26。00%,43。33%,26。67%,23。33%,20。00%,6。67%。

Keywords: Alkylresorcinols; Beauveria bassiana; inhibitory activity

目 录

第一章 绪论 1

1。1  引言 1

1。2  烷基间苯二酚的研究进展 1

1。3  家蚕真菌病及其侵染过程研究进展 5

1。4  家蚕真菌病防治的研究 7

1。4。1  家蚕对病原真菌的抗性机理 7

1。4。2  防僵药剂的研究 7

1。4。3  家蚕真菌病发生及流行规律的研究 8

1。5  研究内容 8

第二章 材料和方法