摘 要本研究通过盆栽试验，考察了叶桑“强桑 1 号”和果桑“无籽大十”对不同土壤 铅胁迫的适应性。结果表明，随着土壤铅浓度的增加，两个品种桑树的生长受到了一 定程度的胁迫，桑树通过提高抗氧化酶活性、增加渗透调节物积累，显著提高了对土 壤铅的适应能力。叶绿素荧光参数和 SPAD 值可以用于评估桑树在土壤铅胁迫下的生 长状况，抗氧化酶超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽还原酶等在 桑树应对土壤铅造成的氧化胁迫中发挥了协同作用，可溶性糖和可溶性蛋白在中低土 壤铅胁迫下发挥了重要的渗透调节功能，在重度土壤铅胁迫下脯氨酸的渗透调节能力 更强。添加外源生物炭可以提高强桑 1 号的叶片含水量、过氧化氢酶、谷胱甘肽还原 酶和还原型谷胱甘肽含量，进而提高了桑树的抗氧化能力。“强桑 1 号”和 “无籽 大十”对土壤铅污染具有较强的适应能力，在 800mg/Kg 的重度土壤铅污染下正常生 长，可用于铅污染耕地栽培种植。80723

毕业论文关键词：土壤铅污染；桑树；生物炭；氧化胁迫；渗透调节

I

Abstract

The study by pot experiment studied the adaptability of Ye Mulberry “QiangSang 1” and Mulberry “Wuzhidashi” to the stress in soil that contained different lead。 The result show that, to a certain degree, the growth of two varieties of Mulberry was stressed with the increase in soil lead concentration。 However, Mulberry greatly improve the adaptability to the lead content soil by increasing antioxidant enzyme activity and osmotic adjustment of accumulation。 Chlorophyll fluorescent parameter and SPAD can assess the growth of Mulberry under stress of lead content soil。 Besides, Anti-oxidation enzyme, Super oxide disambiguation of enzyme, Hydrogen peroxide enzyme, Peroxide enzyme, Glutathione reductase, and so on。 They play a collaborative role in stress that soil lead cause oxidation。 Soluble sugar and soluble protein in the low lead-content soil stress have important function of osmotic regulation。 In addition, soil contains lead in heavy degrees, the capacity of Pro ammonia acid in osmotic regulation is stronger。 Exogenous biochar can raise leaves’ water storage of “QiangSang 1”, and it raise the content of Catalase, Glutathione reductase and reduced Glutathione, and then it improves antioxidant capacity of mulberry trees。 “QiangSang 1” and “Wuzhidashi” both have a strong capacity to adapt the lead pollution in soil, under severe lead pollution in soil in 800mg/Kg, Mulberry can grow normally so that it can be used for planting in the lead contamination of fields。

Key words: Soil Pb contamination; Mulberry; Biochar; Oxidative stress; Osmoregulation

目 录

第一章 绪 论 1

1。1 土壤重金属铅污染现状及植物修复研究进展 1

1。1。1 我国土壤中铅污染的基本现状 1

1。1。2 土壤重金属铅污染对生物的影响 1

1。1。3 植物对土壤铅污染的适应 3

1。1。4 植物修复技术 3

1。1。5 植物修复技术的局限性 4

1。2 桑树在土壤重金属污染的植物修复研究进展 5

1。2。1 桑树的基本生理特征 5

1。2。2 土壤重金属污染对桑树的影响 6

1。2。3 桑树在重金属污染植物修复中的优势