前体和诱导子对桑悬浮细胞中次生物积累的影响
毕业论文关键词：桑树；细胞悬浮培养；次生代谢产物；诱导子
Abstract Mulberry in a variety of secondary metabolites, such as flavonoids, polysaccharides and alkaloids, etc。, have fall blood sugar, anti-oxidation, improving immunity, etc, can be used as drugs raw materials。 Plant cell suspension culture technique can be used to help the plant to break through the limit of its own growth cycle, geographical and environmental factors, to achieve large-scale production of plant cell metabolites。 This experiment in successfully established under the premise of mulberry suspension cell culture system, studies on the different exogenous additives (precursor and inducer) for cell growth and accumulation of flavonoids, polysaccharide and 1-DNJ of mulberry suspension。 The results showed that the exogenous substances showed that all exogenous additives have inhibitory effect on cell growth, which is not conducive to cells suspended biomass accumulation; But for the second biological accumulation show different entrainment。 MJ, SNP, COR and phenylalanine for suspension cells are promoting effect, the accumulation of flavonoids in MJ induced effect is best。 For the accumulation of polysaccharide, only COR showed obvious promotion effect; MJ has induced effect significantly on 1-DNJ in cell lines, and SNP has inhibitory effect on 1-DNJ。 The exogenous additives for cell growth and secondary metabolites synthesis of mulberry suspension show different induction。 Elicitor stimulation of plant cells start different defense reaction, produce different secondary metabolites。
Key words: Mulberry; Cell Suspension Culture; Secondary Metabolites; Elicitor
目录
第一章 绪论 1
1。1 桑的概述 1
1。2 桑树中的活性成分 1
1。3 桑树悬浮细胞培养 2
1。3。1 悬浮细胞培养概述 2
1。3。2 植物悬浮细胞培养的影响因素 3
1。3。2。1 外植体 3
1。3。2。2 愈伤组织 3
1。3。2。3 培养基 3
1。3。2。4 培养条件 4
1。3。3 植物悬浮细胞培养的优点 5
1。3。4 植物悬浮细胞培养的现状 5
1。3。2。1 前体的应用 5
1。3。2。2 诱导子的应用 6
1。4 本文研究内容 6
第二章 实验材料与方法 7
2。1 实验仪器与试剂 7
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