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摘要：本课题主要研究了银耳提取液的制备技术及其应用研究，改变四个影响因素：提取的时间（2h～10h），提取液的pH（4～10），提取的温度（80～100摄氏度），提取液的料液比（1：40～1：120），通过测定银耳提取液的吸光度，并和葡萄糖多糖质量浓度标准曲线对比得到提取液中的多糖质量浓度，从而来得到四个单因素的较优条件,分别为提取的时间为4h，提取液的pH为6，提取的温度为100摄氏度，提取液的料液比为1:80。再进行4因素3水平的正交试验，通过测试提取液的吸光度和葡萄糖多糖质量浓度标准曲线对比得到最大的质量浓度，从而确定最优的银耳提取液的制备工艺因素（提取时间为5h，提取液的pH为6，提取的温度为100摄氏度，提取液的料液比为1：80）。通过优化后制备技术得到的银耳提取液加入到化妆品配方中，通过流变调节剂，保湿剂，水溶性油脂的加入与复配，来降低化妆品在加入银耳提取液后，使用的过程中所会带来的不舒适的黏腻肤感，实验发现可以通过加入降粘效果的原料和加快化妆品的吸收可以达到这个效果。19991
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关键词：银耳提取液；制备；多糖质量浓度；保湿
The preparative technique and applied research of the extracts from tremella
Abstract: In this study, the preparative technique and applied research of the extracts from tremella were studied. By adjust the four influence factors: the time of the extract( from 2 hours to 10 hours), the pH of the extracting solution(from 4 to 10), the temperature of the extract (from 80 degrees Celsius to 100 degrees Celsius), and the ratio of material to solvent (from 1:40 to 1:120). By measuring the absorbance of the extracts from tremella, and contrasting with the canonical plotting, so that we can get the mass concentration of the extracts from tremella. According the mass concentration, the optimum synthesis conditions for the extracts from tremella is that the time of the extract is 4, the temperature of the extract is 100 degrees Celsius and the ratio of material to solvent is 1:80. After that I do the four factors, four levels orthogonal experiment and measure the absorbance of the extract from tremella, the optimal factors is that the time of the extract is 5, the temperature of the extract is 100 degrees Celsius and the ratio of material to solvent is 1:80. At last, by joining the rheolobic modifiers, hydrolyzed glycosaminoglycans and the soluble oil, lower the sticky feeling of the cosmetics. According to the experiment, joining the material with the bate adhesive efficiencyand fasting the rate of absorption can achieve the results.
KeyWords:tremella extract; preparation; mass concentration of polysaccharide
目录
1.绪论 1
1.1 概述 1
1.1.1 天然植物萃取物在化妆品中的应用 1
1.1.2 天然植物萃取物的保湿作用 1
1.1.3 多糖型保湿剂 2
1.1.4 银耳的形态和功效 3
1.1.5 银耳多糖 4
1.1.6 银耳多糖提取工艺 5
1.2 本课题的研究目的和意义 5
2.实验材料与方法 5
2.1 实验材料与试剂 6
2.2 仪器与设备 6
2.3 实验方法 7
2.3.1 水提法工艺流程 7
2.3.2 银耳多糖提取液中银耳多糖质量浓度的测定 7
2.3.3 银耳多糖提取液的粘度测定 8
2.3.4 银耳多糖提取液的流变性测定 8
2.3.5含银耳多糖护肤品的保湿型测定 8
3.实验结果与讨论 9