摘要：以干燥葛仙米为原材料，采用浸提法提取葛仙米多糖，并且通过时间、料液比、温度三个条件的实验，得出结论。实验结果表明，葛仙米多糖的最佳提取条件为：加水溶解料液比为1:500，温度在90℃下搅拌5个小时。并且葛仙米多糖具有抗氧化性、无明显的抑菌效果、还能为皮肤提供保湿作用，所以葛仙米也被应用在化妆品、生物医药、保健品等领域。葛仙米属于典型牛顿流体，它的粘度与温度、PH、浓度等因素有关，所以葛仙米在食品领域的应用也很广。81270

毕业论文关键词： 葛仙米；多糖；提取

Extraction and Application of Polysaccharide from Ge Xian Mi

Abstrcat: The results showed that the polysaccharides were extracted by immersion method, and the results were obtained by three experiments: time, material ratio and temperature。 The results showed that the optimum extraction conditions were as follows: the ratio of water to water was 1: 500, and the temperature was stirred at 90 ℃ for 5 hours。 And Ge Xian Mi polysaccharide with antioxidant, no significant antibacterial effect, but also for the skin to provide moisturizing effect，So Ge Xian Mi is also used in cosmetics, bio-medicine, health care products and other fields。 Ge Xianmi is a typical Newtonian fluid, its viscosity and temperature, PH, concentration and other factors, so the application of rice in the field of food is also very wide。

Keyword：Ge Xian Mi；Polysaccharide；extract

目录

1绪论 1

1。1葛仙米多糖的介绍 1

1。2 国内外对葛仙米多糖的提取研究 1

1。3 课题研究的内容、目的以及意义 2

1。3。1 本课题研究的内容 2

1。3。2 本课题研究的目的和意义 3

2材料与方法 4

2。1 实验原料 4

2。1。1 实验试剂 4

2。1。2 实验器材 4

2。2。1总糖的标准曲线 5

2。2。2 单糖的标准曲线 6

2。3 葛仙米多糖的提取 7

2。3。1 葛仙米多糖提取条件的优化（时间、温度、料液比） 7

2。4。 葛仙米多糖的抗氧化性能测定 8

2。4。1 葛仙米多糖抗氧化性质 8

2。5 葛仙米多糖流变性能的研究方法 9

2。5。1 葛仙米多糖流变性能测定 9

2。6 葛仙米多糖的抑菌效果 9

2。6。1 葛仙米多糖溶液的配制 9

2。6。2 菌种的制备与培养基的制作 9

2。6。3 牛津杯抑菌实验方法 10

2。7 化妆品中的应用 11

2。7。1 葛仙米多糖的提取 11

2。7。2 配方： 11

2。7。3 测试其的保湿性： 11

3。实验结果与讨论 11

3。1 单因素实验对葛仙米多糖的影响 11

3。1。1 不同料液比对葛仙米多糖的影响