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摘要：本文从添加乳双歧杆菌Bi-07、动物双歧杆菌Bb-12以及乳双歧杆菌Bi-07和动物双歧杆菌Bb-12混合的蛋白质营养粉出发，研究了填充氮气，贮藏温度以及不同种类的益生元对三种蛋白质营养粉稳定性的影响。实验以益生菌的活菌量为检测指标，通过厌氧操作定期确定活菌残留数，从而得到最佳的蛋白质营养粉的贮藏条件，并在22℃常温下不填充氮气进行低聚半乳糖，低聚果糖以及两种益生元混合添加对益生菌活性促进效果的研究。结果证明：适宜作为添加乳双歧杆菌Bi-07的蛋白质营养粉储藏条件为22℃且不填充氮气；适宜作为添加动物双歧杆菌Bb-12蛋白质营养粉储藏条件为37℃且填充氮气；适宜作为添加乳双歧杆菌Bi-07和动物双歧杆菌Bb-12混合的蛋白质营养粉储藏条件为22℃且填充氮气。实验通过在营养粉中添加益生元研究益生元对两种菌种及复配菌种的稳定性的影响，结果表明益生元以低聚果糖添加进Bi-07蛋白质营养粉可最好的保持Bi-07益生菌的活性；益生元以低聚乳糖及低聚果糖以1:1复配添加进Bb-12蛋白质营养粉可最好的保持益生菌Bb-12的活性；益生元以低聚半乳糖添加进Bi-07和Bb-12混合蛋白质营养粉可最好的保持Bi-07和Bb-12混合益生菌的活性。48697
毕业论文关键词：益生菌；益生元；活菌数；营养粉；厌氧
The development of promoting the intestinal health nutrition powder
Abstract：From adding milk Bifidobacterium Bi-07, animal Bifidobacterium Bb-12 and dairy Bifidobacterium coli Bi-07 and animal bifid Bacillus Bb-12 mixed protein nutrition powder, this paper studied nitrogen filling, storage temperature, and different kinds of probiotic yuan on the effect of three kinds of protein powder stability. Experimental with live probiotics bacteria as indexes, through anaerobic operation periodically to determine viable residue number, so as to obtain the best protein powder storage conditions, and in 22 degrees under normal temperature without filling nitrogen oligo galactose, fructo oligosaccharide and two kind of prebiotic mixture added to promote the activity of probiotic effect research. Results show that suitable as adding Bifidobacterium lactis Bi-07 of protein nutrition powder storage conditions for 22 degrees and not filled with nitrogen; suitable as adding Bifidobacterium Bb-12 protein powder storage conditions for 37 degrees and nitrogen filling; suitable as add dairy Bifidobacterium coli Bi-07 and animal bifid Bacillus Bb-12 mixed protein nutrition powder storage conditions for 22 degrees and filled with nitrogen.Experiment by adding prebiotics of prebiotics on two strains and mixed strain stability in nutritional powder. The results show that the prebiotic oligofructose added into Bi-07 protein powder can be best to keep Bi-07 probiotic activity; prebiotic oligosaccharides lactose and low fructosan in a 1:1 mixture added into Bb-12 protein powder can be best to preserve the probiotic Bb-12 activity; probiotic yuan to oligo galactose added into the Bi-07 and Bb-12 mixed protein powder can be best to preserve the Bi-07 and Bb-12 mixed probiotic activity.
KeyWords: Probiotics; prebiotics; number of viable bacteria;nutrition power; anaerobic
目录
1绪论 1
1．1 益生菌简介 1
1.2 益生菌在国内外研究进展 2
1.3 益生元简介 2
1.3.1 低聚半乳糖（GOS）的生理功能 3
1.3.2 低聚半乳糖的应用 4
1.3.3 低聚果糖（FOS）的生理功能 4
1.3.4 低聚果糖的应用 5
1.4 本课题研究目的和意义