摘要：选取国内5种知名品牌白酒，利用顶空固相微萃取法（HS-SPME）提取酒样中的挥发性成分，通过气相色谱—质谱联用(GC-MS)技术，分别对5种酒的挥发性成分进行分析，检测出白酒酒样中的挥发性物质。在该条件下5种酒分析出180种挥发性物质，其中酯类物质100种，醇类物质32种，醛类物质14种，酸类物质9种，酮类物质13种，酚类物质4种，烷烃类8种，呋喃类5种，烯烃类2种，含氮化合物2种，含硫化合物1种。研究发现白酒中除酯类、醇类、酸类、醛酮类，酚类、呋喃类等主要的挥发性物质外，还有少量含硫化合物，因此，借助气相色谱-火焰光度检测器（GC-FPD）对含硫化合物检测高效、专一性强的特点，建立了一种简单、快速检测白酒中含硫化合物的方法，利用该方法在5种白酒检测出22种含硫化合物。63210

毕业论文关键词： 白酒；顶空固相微萃取（HS-SPME）；气相色谱-质谱（GC-MS）；挥发性成分；气相色谱-火焰光度检测器（GC-FPD）

Characterization of Volatile Compounds of    Chinese Famous Liquors by HS-SPME-GC-MS and                      GC-FPD

Abstract：In order to research volatile compounds in liquors, 5 Chinese famous liquors had been selected and investigated. The volatile compounds in liquors were extracted by headspace solid-phase microextraction (HS-SPME).And then the volatile compounds were analyzed by gas chromatography-mass spectrometry（GC-MS）. A total of 180 volatile compounds were detected and identified , including 100 esters, 32 alcohols, 14 aldehydes, 9 acids, 13 ketones and 4 phenols, 8 acetals, 5 furfurans, 2 alkenes, 2 nitrogenous compounds, and 1 sulfuric compound. Several sulfur compounds were found in liquors in addition to esters, alcohols, acids, aldehydes and ketones, phenols, furfurans and so on. Therefore, A simple and rapid method to detect sulfur compounds in liquors was established with the help of high efficiency and specificity of gas chromatography-flame photometric detector(GC-FPD) to sulfur compounds. Moreover, a total of 22 sulfur compounds in five liquors were detected.

KeyWords: Chinese liquors; HS-SPME; GC-MS; volatile compounds; GC-FPD

目  录

1  绪论 1

1.1  白酒概述 1

1.2  白酒的研究现状 2

1.3  白酒挥发性成分的分析方法 4

1.3.1  顶空固相微萃取（HS-SPME）技术 4

1.3.2  气相色谱-质谱连用（GC-MS）技术 5

1.3.3  气相色谱-火焰光度检测器（GC-FPD）检测技术 6

1.4  本课题的研究目的和意义 7

2  实验部分 8

2.1  原料、试剂和仪器设备 8

2.1.1  实验原料 8

2.1.2  实验试剂 8

2.1.3  实验仪器设备 8

2.2  样品处理方法和分析条件 8

2.2.1  顶空固相微萃取（HS-SPME） 8

2.2.2 气相色谱-质谱连用（GC-MS） 9

2.2.3  气相色谱-火焰光度检测器（GC-FPD） 9

2.3挥发性化合物定性分析 9

2.4挥发性化合物的定量方法