银纳米颗粒对血液荧光增强效果的时间作用研究
毕业论文关键词：血液 银纳米颗粒 荧光增强 作用时间
Study on Time Effect of Silver Nanoparticles on the Fluorescence Enhancement of Bloods
Abstract: Time effect of silver nanoparticles on the fluorescence enhancement of bloods was investigated by the FLS900 steady-state and transient fluorescence spectrometer in this paper. The fluorescence spectrum of whole blood solution and whole blood of silver sol solution are investigated experimentally by time,and the fluorescence intensity and fluorescence enhancement ratio of each fluorescence emission peak in the spectrum are analyzed theoretically. The result showed：in the blood of the silver nanoparticles occurs fluorescence enhancement effect with flavin adenine dinucleotide, blood porphyrin rings, and zinc porphyrin ,in wavelength of 309 nm, 560 nm and 593 nm in the fluorescence spectrum; fluorescence enhancement phenomenon occurs within 2 hours after completion of the sample preparation but 3 hours after ,it significantly reduced; the fluorescence enhancement effect of Flavin adenine dinucleotide is best 2 hours later after sample preparation, blood porphyrin rings, zinc porphyrin achieve the biggest fluorescence enhancement ratio just after the completion of preparation. After discussing the mechanism of the fluorescence enhancement effect, it is considered that the different distance between silver nanoparticles and fluorescent material in whole blood solution in each period after completion of the preparation influence the strength of fluorescence enhancement effect. The research in this paper can offer reference to the study of fluorescence spectrum about flavin adenine dinucleotide, porphyrin rings and zinc porphyrin and their application in clinical diagnosis, and also provides the case for research in other fluorescence spectrum which discusses time effect.
Keywords: blood, silver nanoparticles, fluorescence enhancement, time effect
目录
摘要 i
Abstract. ii
1 绪论 1
2 荧光光谱理论 2
2.1 荧光光谱的相关知识 2
2.2 血液荧光光谱 4
3 金属纳米粒子的荧光增强效应 6
4 实验材料与方法 9
4.1 实验材料 9
4.2 制备方法 9
4.2 实验仪器 10
4.2 实验方法 10
5 实验结果 11
5.1 全血溶液荧光光谱随时间的变化规律研究 11
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